Dust from carpeted and smooth floors. V. Cat (Fel d I) and mite (Der p I and Der f I) allergen levels in school dust. Demonstration of the basophil histamine release induced by dust from classrooms
- 1 December 1992
- journal article
- Published by Wiley in Clinical and Experimental Allergy
- Vol. 22 (12), 1100-1106
- https://doi.org/10.1111/j.1365-2222.1992.tb00136.x
Abstract
Sixty dust samples from schools in Norway were analysed for major allergens from cat and mite after sampling with the regularly used vacuum cleaners for 5 days and with a new model vacuum cleaner for 10 days, respectively. The major feline allergen Felis domesticus allergen I (Fel d I) was detected in all the classrooms, with ranges from 12 to 16,840 ng/m2 floor area. The mean Fel d I concentration was about 11 times higher per unit area carpeted floors as compared with smooth floors after the 10 days sampling period. Mite allergens Dermatophagoides pteronyssinus allergen I (Der p I) and Dermatophagoides farinae allergen I (Der f I) were detected in very low concentrations, with ranges from < 1 ng to 104 ng/m2 floor area. These findings suggest that the school is a protective environment against mite infestation, while the prevalence of cat allergens in classrooms seems higher than previously assumed. Basophil histamine release was measured after provocation with 20 dust extracts from 10 different schools. In nine of the 10 schools examined, the basophil histamine release caused by challenge with carpet dust was higher than the corresponding release with smooth floor dust. The calculated floor areas in each school in which dust led to 15% histamine release were from 2 to 55 times larger for smooth floors compared with carpeted floors. These results emphasize previous findings regarding higher allergen concentrations in classrooms with carpeted floors.Keywords
This publication has 13 references indexed in Scilit:
- Dust from carpeted and smooth floors: IV. Solid material, proteins and allergens collected in the different filter stages of vacuum cleaners after ten days of use in schools.Allergy, 1991
- Dust from carpeted and smooth floors: IV. Solid material, proteins and allergens collected in the different filter stages of vacuum cleaners after ten days of use in schools.Allergy, 1991
- An immunoassay for histamine based on monoclonal antibodiesJournal of Immunological Methods, 1990
- Dust from carpeted and smooth floorsAllergy, 1989
- The effect of cat removal on allergen content in household-dust samplesJournal of Allergy and Clinical Immunology, 1989
- A two-site monoclonal antibody ELISA for the quantification of the major Dermatophagoides spp. allergens, Der p I and Der f IJournal of Immunological Methods, 1989
- Dust from carpeted and smooth floors. I. Comparative measurements of antigenic and allergenic proteins in dust vacuumed from carpeted and non‐carpeted classrooms in Norwegian schoolsClinical and Experimental Allergy, 1989
- Monoclonal immunoassay for major dust mite (Dermatophageides) allergens, Derp I and Derf I, and quantitative analysis of the allergen content of mite and house dust extractsJournal of Allergy and Clinical Immunology, 1987
- Recognition of two Dermatophagoides pteronyssinus-specific epitopes on antigen P1 by using monoclonal antibodies: binding to each epitope can be inhibited by serum from dust mite-allergic patients.The Journal of Immunology, 1984
- Dissociation of IgE from receptors on human basophils. I. Enhanced passive sensitization for histamine release.The Journal of Immunology, 1983