DNA Extraction from Formalin-fixed, Paraffin-embedded Tissues
- 1 December 1997
- journal article
- Published by Ovid Technologies (Wolters Kluwer Health) in Diagnostic Molecular Pathology
- Vol. 6 (6), 342-346
- https://doi.org/10.1097/00019606-199712000-00006
Abstract
Several DNA extraction methods have been used for formalin-fixed, paraffin-embedded tissues, with variable results being reported regarding the suitability of DNA obtained from such sources to serve as template in polymerase chain reaction (PCR)-based genetic analyses. We present a method routinely used for archival material in our laboratory that reliably yields DNA of sufficient quality for PCR studies. This method is based on extended proteinase K digestion (250 micrograms/ml in an EDTA-free calcium-containing buffer supplemented with mussel glycogen) followed by phenol-chloroform extraction. Agarose gel electrophoresis of both digestion buffer aliquots and PCR amplification of the beta-globin gene tested the suitability of the retrieved DNA for PCR amplification.Keywords
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