Analysis on DNA sequence of KiSS-1 gene and its association with litter size in goats
- 20 March 2010
- journal article
- research article
- Published by Springer Science and Business Media LLC in Molecular Biology Reports
- Vol. 37 (8), 3921-3929
- https://doi.org/10.1007/s11033-010-0049-7
Abstract
Three pairs of primers were designed to clone the goat KiSS-1 and scan polymorphisms and four pairs to detect polymorphisms in sexual precocious and sexual late-maturing goat breeds. A 4118 bp DNA fragment was obtained, which contains an ORF of 408 bp and encodes 135 amino acids, having a high homology with other mammals. The protein was predicted containing a signal peptide of 17 amino acids. There are two mutations (G3433A [A86T] and C3688A) in exon 3, three mutations (G296C, G454T and T505A) in intron 1 and a 18 bp deletion (−)/insertion (+) (1960–1977) in intron 2 and no mutations in exon 2. The genotype distribution didn’t show obvious difference between sexual precocious and sexual late-maturing goat breeds and no consistency within the sexual late-maturing breeds. For the 296 locus, the Jining Grey goats with genotype CC had 0.80 (P < 0.01) or 0.77 (P < 0.01) kids more than those with genotype GG or GC, respectively. No significant difference (P > 0.05) was found in litter size between GG and GC. For the 1960–1977 locus, the Jining Grey goat does with genotype −/− had 0.77 (P < 0.01) or 0.73 (P < 0.01) kids more than those with +/+ or +/−, respectively. No significant difference (P > 0.05) was found in litter size between +/+ and +/− genotypes. For the other four loci, no significant difference (P > 0.05) was found in litter size between different genotypes in Jining Grey goats. The present study preliminarily indicated an association between allele C of the 296 locus and allele (−) of the 1960–1977 locus in KiSS-1 and high litter size in Jining Grey goats.Keywords
This publication has 39 references indexed in Scilit:
- Characteristics of stimulation of gonadotropin secretion by kisspeptin-10 in female goatsAnimal Reproduction Science, 2009
- Analysis of caprine pituitary specific transcription factor-1 gene polymorphism in indigenous Chinese goatsMolecular Biology Reports, 2008
- Kisspeptin Mediates the Photoperiodic Control of Reproduction in HamstersCurrent Biology, 2006
- The roles of kisspeptins and G protein-coupled receptor-54 in pubertal developmentCurrent Opinion in Pediatrics, 2006
- GPR54 and kisspeptin in reproductionHuman Reproduction Update, 2006
- Repetitive Activation of Hypothalamic G Protein-Coupled Receptor 54 with Intravenous Pulses of Kisspeptin in the Juvenile Monkey (Macaca mulatta) Elicits a Sustained Train of Gonadotropin-Releasing Hormone DischargesEndocrinology, 2006
- Differential Regulation of KiSS-1 mRNA Expression by Sex Steroids in the Brain of the Male MouseEndocrinology, 2005
- Effects of KiSS-1 Peptide, the Natural Ligand of GPR54, on Follicle-Stimulating Hormone Secretion in the RatEndocrinology, 2005
- Kisspeptin directly stimulates gonadotropin-releasing hormone release via G protein-coupled receptor 54Proceedings of the National Academy of Sciences of the United States of America, 2005
- Mutations in the Genes for Oocyte-Derived Growth Factors GDF9 and BMP15 Are Associated with Both Increased Ovulation Rate and Sterility in Cambridge and Belclare Sheep (Ovis aries)1Biology of Reproduction, 2004