RNA Interference—A Silent but an Efficient Therapeutic Tool
- 23 January 2013
- journal article
- review article
- Published by Springer Science and Business Media LLC in Applied Biochemistry and Biotechnology
- Vol. 169 (6), 1774-1789
- https://doi.org/10.1007/s12010-013-0098-1
Abstract
RNA interference (RNAi) is an evolutionary conserved gene regulation pathway that has emerged as an important discovery in the field of molecular biology. One of the important advantages of RNAi in therapy is that it brings about efficient downregulation of gene expression by targeting complementary transcripts in comparison with other antisense-based techniques. RNAi can be can be achieved by introducing chemically synthesized small interfering RNAs (siRNAs) into a cell system. A more stable knockdown effect can be brought about by the use of plasmid or viral vectors encoding the siRNA. RNAi has been used in reverse genetics to understand the function of specific genes and also as a therapeutic tool in treating human diseases. This review provides a brief insight into the therapeutic applications of RNAi against debilitating diseases.Keywords
This publication has 137 references indexed in Scilit:
- Systemic delivery of siRNA nanoparticles targeting RRM2 suppresses head and neck tumor growthJournal of Controlled Release, 2012
- Ocular neuroprotection by siRNA targeting caspase-2Cell Death & Disease, 2011
- Recent Advances in Lentiviral Vector Development and ApplicationsMolecular Therapy, 2010
- Small silencing RNAs: State-of-the-artAdvanced Drug Delivery Reviews, 2009
- AAV8-mediated Gene Transfer of Interleukin-4 to Endogenous β-Cells Prevents the Onset of Diabetes in NOD MiceMolecular Therapy, 2008
- Sequence- and target-independent angiogenesis suppression by siRNA via TLR3Nature, 2008
- Matrices and scaffolds for DNA delivery in tissue engineeringAdvanced Drug Delivery Reviews, 2007
- The Microprocessor complex mediates the genesis of microRNAsNature, 2004
- Effective expression of small interfering RNA in human cellsNature Biotechnology, 2002
- U6 promoter–driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cellsNature Biotechnology, 2002