Cylindrocladium buxicola, a new species affectingBuxusspp., and its phylogenetic status
- 1 November 2002
- journal article
- research article
- Published by Informa UK Limited in Mycologia
- Vol. 94 (6), 980-997
- https://doi.org/10.1080/15572536.2003.11833155
Abstract
A leaf and twig blight disease of Buxus spp. was found to be associated with a new species of Cylindrocladium. The novel species status was confirmed using morphological characters, sequencing of the ribosomal 5.8S RNA gene and the flanking internal transcribed spacers (ITS), the β-tubulin gene, and the high mobility group (HMG) of the MAT2 mating type gene. Cylindrocladium buxicola is proposed as a new name. Fifteen isolates from the UK and one isolate from New Zealand were paired in all combinations but no fertile perithecia were obtained suggesting that C. buxicola is heterothallic and all isolates belonged to one mating type. AFLP analysis showed that the isolates collected in the UK and New Zealand are genetically homogenous. Phylogenetic analyses indicated that this species falls within a new lineage.Keywords
This publication has 26 references indexed in Scilit:
- Phylogenetic relationships of Cylindrocladium pseudogracile and Cylindrocladium rumohrae with morphologically similar ta×a, based on morphology and DNA sequences of internal transcribed spacers and beta-tubulinCanadian Journal of Botany, 1999
- New foliar pathogens of Eucalyptus from Australia and IndonesiaMycological Research, 1998
- Delineation of Cylindrocladium species with 1-3-septate conidia and clavate vesicles based on morphology and rDNA RFLPsMycological Research, 1997
- Nuclear DNA Polymorphisms of Cylindrocladium Species with 1-septate Conidia and Clavate VesiclesSystematic and Applied Microbiology, 1995
- Cylindrocladium parasiticum sp. nov., a new name for C. crotalariaeMycological Research, 1993
- Characterization of Some Cylindrocladium Species with Three-Septate Conidia Using Morphology, Isozyme Banding Patterns and DNA PolymorphismsSystematic and Applied Microbiology, 1993
- Calonectria scoparia and Calonectria morganii sp. nov., and variation among isolates of their Cylindrocladium anamorphsMycological Research, 1993
- Effects of Cultural Conditions on Vesicle and Conidium Morphology in Species of Cylindrocladium and CylindrocladiellaMycologia, 1992
- Fungal Molecular SystematicsAnnual Review of Ecology and Systematics, 1991
- Cylindrocladium ilicicola on cuttings of evergreen ornamental shrubs in the UKPlant Pathology, 1987