Restricted access boronate affinity porous monolith as a protein A mimetic for the specific capture of immunoglobulin G
- 22 February 2012
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Chemical Science
- Vol. 3 (5), 1467-1471
- https://doi.org/10.1039/c2sc20125a
Abstract
Antibodies are molecular workhorses in biological research, disease treatment and diagnostics. Purity is a critical prerequisite for antibody applications. Although protein A-based affinity chromatography has developed into the gold standard for antibody purification, protein A is associated with several apparent disadvantages, including high cost, poor stability and harsh product release conditions. Many attempts have been made towards molecular level biomimetics of protein A. However, practical substitutes have not yet been achieved. Here we present a novel functionalized material, called restricted access boronate affinity porous monolith, as a mimic of protein A for the specific capture of antibodies. This biomimetic relies on a novel strategy that combines the steric hindrance of the porous monolith with the chemical selectivity of boronic acid. This protein A biomimetic material demonstrated high specificity for antibodies. Meanwhile, original immunoaffinity and specificity of the captured antibodies were maintained. Compared with protein A, the monolithic biomimetic exhibited several significant advantages, including low cost, high stability and fast elution kinetics.Keywords
This publication has 48 references indexed in Scilit:
- High‐Capacity Hierarchically Imprinted Polymer Beads for Protein Recognition and CaptureAngewandte Chemie-International Edition, 2010
- Capture of human monoclonal antibodies from a clarified cell culture supernatant by phenyl boronate chromatographyJournal of Molecular Recognition, 2010
- Analysis of protein glycation using phenylboronate acrylamide gel electrophoresisProteomics, 2009
- Synthesis and application of novel phenylboronate affinity materials based on organic polymer particles for selective trapping of glycoproteinsJournal of Separation Science, 2009
- Polymer‐based monolithic columns in capillary format tailored by using controlled in situ polymerizationJournal of Separation Science, 2009
- Boronate affinity saccharide electrophoresis: A novel carbohydrate analysis toolElectrophoresis, 2008
- Protein A‐based antibody immobilization onto polymeric microdevices for enhanced sensitivity of enzyme‐linked immunosorbent assayBiotechnology & Bioengineering, 2008
- Boronic Acid Based Peptidic Receptors for Pattern-Based Saccharide Sensing in Neutral Aqueous Media, an Application in Real-Life SamplesJournal of the American Chemical Society, 2007
- Engineering novel binding proteins from nonimmunoglobulin domainsNature Biotechnology, 2005
- New Designs of Macroporous Polymers and Supports: From Separation to BiocatalysisScience, 1996