Development and application of a novel and effective screening method for aerobic denitrifying bacteria
Open Access
- 1 July 2006
- journal article
- research article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 260 (2), 150-155
- https://doi.org/10.1111/j.1574-6968.2006.00306.x
Abstract
Herein we describe a novel and effective screening method for aerobic denitrifying bacteria. For this procedure, we utilized KCN to inhibit the electron transference from Cytaa3 to oxygen in the bacteria respiratory chain. We employed a 3-h aeration operation cycle and intermittent rotations. The resultant bacterial suspensions were plated on a KCN-screening medium and incubated aerobically. Single colonies were selected and incubated in an aerobic culture medium. Culture nitrate and nitrite levels were determined over time, and ultimately four bacterial strains that performed denitrifying under aerobic conditions were identified by this method. Of these, strain Y2-1-1 demonstrated the best aerobic denitrifying ability. In a 5-day test, the NO3−-N of the aerobic culture medium was reduced from 282.0±8.3mgL−1 to 149.2±17.1mgL−1, with little nitrite or N2O production. The morphological, physiological and biochemical characteristics and the 16S rRNA gene sequence homology comparison data for this strain were consistent with the classification of the genus Pseudomonas. We named this strain Pseudomonas sp. Y2-1-1.Keywords
This publication has 12 references indexed in Scilit:
- Aerobic and Anaerobic Toluene Degradation by a Newly Isolated Denitrifying Bacterium, Thauera sp. Strain DNT-1Applied and Environmental Microbiology, 2004
- Aerobic Denitrification ofPseudomonas aeruginosaMonitored by Online NAD(P)H FluorescenceApplied and Environmental Microbiology, 2003
- Comparison of aerobic denitrification under high oxygen atmosphere by Thiosphaera pantotropha ATCC 35512 and Pseudomonas stutzeri SU2 newly isolated from the activated sludge of a piggery wastewater treatment systemJournal of Applied Microbiology, 2001
- Novel principles in the microbial conversion of nitrogen compoundsAntonie van Leeuwenhoek, 1997
- Insertion of transposon Tn5 into a structural gene of the membrane-bound nitrate reductase of Thiosphaera pantotropha results in anaerobic overexpression of periplasmic nitrate reductase activityJournal of General Microbiology, 1993
- Production of nitric oxide in Nitrosomonas europaea by reduction of nitriteArchiv für Mikrobiologie, 1990
- Periplasmic and membrane‐bound respiratory nitrate reductases in Thiosphaera pantotrophaFEBS Letters, 1990
- Thiosphaera pantotropha gen. nov. sp. nov., a Facultatively Anaerobic, Facultatively Autotrophic Sulphur BacteriumMicrobiology, 1983
- Dissimilatory Nitrate and Nitrite Reduction under Aerobic Conditions by an Aerobically and Anaerobically Grown Alcaligenes sp. and by Activated SludgeJournal of Applied Bacteriology, 1976
- THE THIOBACILLI, 12Microbiology and Molecular Biology Reviews, 1957