Identification of novel proteins and phosphorylation sites in a tonoplast enriched membrane fraction of Arabidopsis thaliana
- 27 August 2008
- journal article
- Published by Wiley in Proteomics
- Vol. 8 (17), 3536-3547
- https://doi.org/10.1002/pmic.200701104
Abstract
Plant vacuoles play essential roles in many physiological processes, particularly in mineral nutrition, turgor provision and cellular signalling. The vacuolar membrane, the tonoplast, contains many membrane transporters that are critical in the execution of these processes. However, although increasing knowledge is available about the identity of proteins involved in these processes very little is known about the regulation of tonoplast transporters. By studying the phosphoproteome of tonoplast‐enriched membranes, we identified 66 phosphorylation sites on 58 membrane proteins. Amongst these, 31 sites were identified in 28 membrane transporters of various families including tonoplast anion transporters of the CLC family, potassium transporters of the KUP family, tonoplast sugar transporters and ABC transporters. In a number of cases, the detected sites were well conserved across isoforms of one family pointing to common mechanisms of regulation. In other cases, isoform‐unique sites were present, suggesting regulatory mechanisms tailored to the function of individual proteins. These results provide the basis for future studies to elucidate the mechanistic regulation of tonoplast membrane transporters.Keywords
This publication has 43 references indexed in Scilit:
- Novel Tonoplast Transporters Identified Using a Proteomic Approach with Vacuoles Isolated from Cauliflower BudsPlant Physiology, 2007
- Quantitative phosphoproteomic analysis of plasma membrane proteins reveals regulatory mechanisms of plant innate immune responsesThe Plant Journal, 2007
- The two-pore channel TPK1 gene encodes the vacuolar K + conductance and plays a role in K + homeostasisProceedings of the National Academy of Sciences, 2007
- Phosphoproteomic identification of targets of the Arabidopsis sucrose nonfermenting-like kinase SnRK2.8 reveals a connection to metabolic processesProceedings of the National Academy of Sciences, 2007
- A Proteomics Dissection of Arabidopsis thaliana Vacuoles Isolated from Cell CultureMolecular & Cellular Proteomics, 2007
- Identification and functional expression of the Arabidopsis thaliana vacuolar glucose transporter 1 and its role in seed germination and floweringProceedings of the National Academy of Sciences, 2007
- A cytosolic trans-activation domain essential for ammonium uptakeNature, 2007
- SUBA: the Arabidopsis Subcellular DatabaseNucleic Acids Research, 2006
- Identification of a Vacuolar Sucrose Transporter in Barley and Arabidopsis Mesophyll Cells by a Tonoplast Proteomic ApproachPlant Physiology, 2006
- Proteomics of the rice cell: systematic identification of the protein populations in subcellular compartmentsMolecular Genetics and Genomics, 2004