Identification of IGPR-1 as a novel adhesion molecule involved in angiogenesis
Open Access
- 1 May 2012
- journal article
- Published by American Society for Cell Biology (ASCB) in Molecular Biology of the Cell
- Vol. 23 (9), 1646-1656
- https://doi.org/10.1091/mbc.e11-11-0934
Abstract
Angiogenesis—the growth of new blood vessels from preexisting vessels—is an important physiological process and is considered to play a key role in tumor growth and metastasis. We identified the immunoglobulin-containing and proline-rich receptor-1 (IGPR-1, also called TMIGD2) gene as a novel cell adhesion receptor that is expressed in various human organs and tissues, mainly in cells with epithelium and endothelium origins. IGPR-1 regulates cellular morphology, homophilic cell aggregation, and cell–cell interaction. IGPR-1 activity also modulates actin stress fiber formation and focal adhesion and reduces cell migration. Silencing of expression of IGPR-1 by small interfering RNA (siRNA) and by ectopic overexpression in endothelial cells showed that IGPR-1 regulates capillary tube formation in vitro, and B16F melanoma cells engineered to express IGPR-1 displayed extensive angiogenesis in the mouse Matrigel angiogenesis model. Moreover, IGPR-1, through its proline-rich cytoplasmic domain, associates with multiple Src homology 3 (SH3)–containing signaling proteins, including SH3 protein interacting with Nck (SPIN90/WISH), bullous pemphigoid antigen-1, and calcium channel β2. Silencing of expression of SPIN90/WISH by siRNA in endothelial cells showed that SPIN90/WISH is required for capillary tube formation. These features of IGPR-1 suggest that IGPR-1 is a novel receptor that plays an important role in cell–cell interaction, cell migration, and angiogenesis.Keywords
This publication has 31 references indexed in Scilit:
- Molecular mechanisms and clinical applications of angiogenesisNature, 2011
- PEST Motif Serine and Tyrosine Phosphorylation Controls Vascular Endothelial Growth Factor Receptor 2 Stability and DownregulationMolecular and Cellular Biology, 2011
- c-Cbl inhibits angiogenesis and tumor growth by suppressing activation of PLCγ1Oncogene, 2011
- Vascular endothelial platelet endothelial cell adhesion molecule 1 (PECAM-1) regulates advanced metastatic progressionProceedings of the National Academy of Sciences of the United States of America, 2010
- IQGAP1-Dependent Signaling Pathway Regulates Endothelial Cell Proliferation and AngiogenesisPLOS ONE, 2008
- Nectins and nectin-like molecules: roles in contact inhibition of cell movement and proliferationNature Reviews Molecular Cell Biology, 2008
- Dscam and Sidekick proteins direct lamina-specific synaptic connections in vertebrate retinaNature, 2008
- Dynamics of cell adhesion and motility in living cells is altered by a single amino acid change in E‐cadherin fused to enhanced green fluorescent proteinCell Motility, 2004
- Dynamic interaction of VCAM-1 and ICAM-1 with moesin and ezrin in a novel endothelial docking structure for adherent leukocytesThe Journal of cell biology, 2002
- Bridging cytoskeletal intersectionsJournal of Bone and Joint Surgery, 2001