Mouse fertility is enhanced by oocyte‐specific loss of core 1‐derived O‐glycans
Open Access
- 11 February 2008
- journal article
- research article
- Published by Wiley in The FASEB Journal
- Vol. 22 (7), 2273-2284
- https://doi.org/10.1096/fj.07-101709
Abstract
Regulation of the number of eggs ovulated by different mammalian species remains poorly understood. Here we show that oocyte‐specific deletion at the primary follicle stage of core 1 β 1,3‐galactosyl‐transferase (T‐synthase;generates core 1‐derived O‐glycans), leads to a sustained increase in fertility. T‐syn mutant females ovulated 30–50% more eggs and had a sustained increase in litter size compared to controls. Ovarian weights and follicle numbers were greater in mutants, but follicular apoptosis was not decreased. The number of follicles entering the growing pool was unaltered, but 3‐wk mutants ovulated fewer eggs, suggesting that increased fertility results from prolonged follicle development. T‐syn mutant ovaries also contained numerous multiple‐oocyte follicles (MOFs) that appeared to form by adjacent, predominantly preantral, follicles joining — a new mechanism for MOF generation. Ovulation of multiple eggs from MOFs was not the reason for increased fertility based on ovulated egg and corpora lutea numbers. Thus, the absence of T‐synthase caused modified follicular development, leading to the maturation and ovulation of more follicles, to MOF formation at late stages of folliculogenesis, and to increased fertility. These results identify novel roles for glycoproteins from the oocyte as suppressors of fertility and regulators of follicular integrity in the mouse.—Williams, S. A., Stanley, P. Mouse fertility is enhanced by oocyte‐specific loss of core 1‐ derived O‐glycans. FASEB J. 22, 2273–2284 (2008)Keywords
Funding Information
- National Cancer Institute (RO1 30645)
- Albert Einstein Cancer Center (PO1 13330)
This publication has 52 references indexed in Scilit:
- CPEB controls oocyte growth and follicle development in the mouseDevelopment, 2006
- Extracellular Matrix of the Developing Ovarian FollicleSeminars in Reproductive Medicine, 2006
- Ovarian follicle development and transgenic mouse modelsHuman Reproduction Update, 2006
- Inactivation of the Mgat1 Gene in Oocytes Impairs Oogenesis, but Embryos Lacking Complex and Hybrid N-Glycans Develop and ImplantMolecular and Cellular Biology, 2004
- Defective angiogenesis and fatal embryonic hemorrhage in mice lacking core 1–derived O-glycansThe Journal of cell biology, 2004
- Infertility in female mice with an oocyte-specific knockout of GPI-anchored proteinsJournal of Cell Science, 2003
- Developmental and Molecular Aberrations Associated with Deterioration of Oogenesis During Complete or Partial Follicle-Stimulating Hormone Receptor Deficiency in MiceBiology of Reproduction, 2003
- Mutant female mice carrying a singlemZP3allele produce eggs with a thin zona pellucida, but reproduce normallyProceedings. Biological sciences, 1997
- O-Linked Trisaccharide and N-Linked Poly-N-Acetyllactosaminyl Glycans are Present on Mouse ZP2 and ZP31Biology of Reproduction, 1994
- The Effects of an Aromatase Inhibitor and a 5α-Reductase Inhibitor upon the Occurrence of Polyovular Follicles, Persistent Anovulation, and Permanent Vaginal Stratification in Mice Treated Neonatally with Testosterone1Biology of Reproduction, 1988