Fabrication and surface modification of macroporous poly(L‐lactic acid) and poly(L‐lactic‐co‐glycolic acid) (70/30) cell scaffolds for human skin fibroblast cell culture
- 22 August 2002
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research
- Vol. 62 (3), 438-446
- https://doi.org/10.1002/jbm.10318
Abstract
The fabrication and surface modification of a porous cell scaffold are very important in tissue engineering. Of most concern are high-density cell seeding, nutrient and oxygen supply, and cell affinity. In the present study, poly(L-lactic acid) and poly(L-lactic-co-glycolic acid) (70/30) cell scaffolds with different pore structures were fabricated. An improved method based on Archimedes' Principle for measuring the porosity of scaffolds, using a density bottle, was developed. Anhydrous ammonia plasma treatment was used to modify surface properties to improve the cell affinity of the scaffolds. The results show that hydrophilicity and surface energy were improved. The polar N-containing groups and positive charged groups also were incorporated into the sample surface. A low-temperature treatment was used to maintain the plasma-modified surface properties effectively. It would do help to the further application of plasma treatment technique. Cell culture results showed that pores smaller than 160 μm are suitable for human skin fibroblast cell growth. Cell seeding efficiency was maintained at above 99%, which is better than the efficiency achieved with the common method of prewetting by ethanol. The plasma-treatment method also helped to resolve the problem of cell loss during cell seeding, and the negative effects of the ethanol trace on cell culture were avoided. The results suggest that anhydrous ammonia plasma treatment enhances the cell affinity of porous scaffolds. Mass transport issues also have been considered. © 2002 Wiley Periodicals, Inc. J Biomed Mater Res 62: 438–446, 2002Keywords
This publication has 38 references indexed in Scilit:
- Physico-chemistry of initial microbial adhesive interactions – its mechanisms and methods for studyFEMS Microbiology Reviews, 1999
- Physico-chemistry of initial microbial adhesive interactions – its mechanisms and methods for studyFEMS Microbiology Reviews, 1999
- Synthetic Extracellular Matrices for Cell TransplantatonMaterials Science Forum, 1997
- Design of Macroporous Biodegradable Polymer Scaffolds for Cell TransplantationMaterials Science Forum, 1997
- Synthesis and RGD peptide modification of a new biodegradable copolymer: poly(lactic acid-co-lysine)Journal of the American Chemical Society, 1993
- Tissue EngineeringScience, 1993
- Laminated three-dimensional biodegradable foams for use in tissue engineeringBiomaterials, 1993
- Changes in wettability with time of plasma-modified perfluorinated polymersJournal of Adhesion Science and Technology, 1992
- Surface properties of commercial polymer films following various gas plasma treatmentsJournal of Applied Polymer Science, 1991
- Protein adsorption at the interface between charged polymer substrata and migrating osteoblastsBiomaterials, 1988