Porcine reproductive and respiratory syndrome virus (PRRSV) infection status in pigs naturally affected with post-weaning multisystemic wasting syndrome (PMWS) in Spain
- 26 February 2002
- journal article
- Published by Elsevier BV in Veterinary Microbiology
- Vol. 85 (1), 23-30
- https://doi.org/10.1016/s0378-1135(01)00474-6
Abstract
The purpose of the study reported here was to determine the prevalence of porcine reproductive and respiratory syndrome virus (PRRSV) in pigs affected with post-weaning multisystemic wasting syndrome (PMWS), a disease believed to be caused by porcine circovirus type 2 (PCV2). From May 1997 to February 2000, PMWS was diagnosed in 277 pigs (from 120 farms) submitted to the Veterinary Pathology Diagnostic Service, Veterinary School of Barcelona, Spain. In each case, the PMWS diagnosis was based on clinical history and the detection, by in situ hybridization, of nucleic acid of PCV2 in characteristic histologic lesions. Antigens for PRRSV were detected by immunohistochemistry in tissues of 66 (23.8%) of the same 277 pigs. Sera, which were available for 93 of the 277 pigs, were tested for PRRSV by a multiplex reverse transcription–polymerase chain reaction (RT–PCR). A total of 33 of these sera were RT–PCR positive, three for a North American strain(s) of PRRSV. In addition, 76 of the 93 sera were tested for antibodies to PCV2 (indirect immunoperoxidase) and PRRSV (enzyme-linked immunoassay). Antibodies for PCV2 and PRRSV were detected, respectively, in 56 (73.9%) and 43 (56.6%) of the 76 sera. Collectively, these results suggest that while infection with PRRSV may be common, it is not an essential component of PMWS.Keywords
This publication has 15 references indexed in Scilit:
- Activation of the Immune System is the Pivotal Event in the Production of Wasting Disease in Pigs Infected with Porcine Circovirus-2 (PCV-2)Veterinary Pathology, 2001
- Experimental infection of colostrum deprived piglets with porcine circovirus 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) potentiates PCV2 replicationArchiv für die gesamte Virusforschung, 2000
- Viral Wasting Syndrome of Swine: Experimental Reproduction of Postweaning Multisystemic Wasting Syndrome in Gnotobiotic Swine by Coinfection with Porcine Circovirus 2 and Porcine ParvovirusVeterinary Pathology, 2000
- Porcine Circoviruses: A ReviewJournal of Veterinary Diagnostic Investigation, 2000
- Coinfection by Porcine Circoviruses and Porcine Parvovirus in Pigs with Naturally Acquired Postweaning Multisystemic Wasting SyndromeJournal of Veterinary Diagnostic Investigation, 2000
- Reproduction of Lesions of Postweaning Multisystemic Wasting Syndrome by Infection of Conventional Pigs with Porcine Circovirus Type 2 Alone or in Combination with Porcine ParvovirusJournal of Comparative Pathology, 2000
- Experimental Inoculation of Conventional Pigs with Tissue Homogenates from Pigs with Post-weaning Multisystemic Wasting SyndromeJournal of Comparative Pathology, 1999
- Experimental Reproduction of Severe Wasting Disease by Co-infection of Pigs with Porcine Circovirus and Porcine ParvovirusJournal of Comparative Pathology, 1999
- Letter to the EditorVeterinary Pathology, 1999
- Development of a Streptavidin-Biotin Immunoperoxidase Procedure for the Detection of Porcine Reproductive and Respiratory Syndrome Virus Antigen in Porcine LungJournal of Veterinary Diagnostic Investigation, 1994