Physical Ordering of Three Polymorphic DNA Markers Spanning the Regions Containing a Tumor Suppressor Gene of Renal Cell Carcinoma by Three-color Fluorescentin situHybridization

Abstract

Fluorescent in situ hybridization (FISH) is a powerful technique for gene mapping and multi‐color FISH allows us to determine directly the order of two or more probes on both metaphase and interphase chromosomes. We report the physical ordering of three DNA markers by three‐color FISH using two fluorochrome dyes, fluorescein isothiocyanate (FITC; green) and rhodamine (red). The third color was visualized as a pseudo‐color (yellow) generated by optical interference with FITC and rhodamine. Using this system we could rapidly determine the order of three polymorphic DNA markers located on the 3p23–p21.2 bands which span a tumor suppressor gene for renal cell carcinoma, lung carcinoma, and several other types of tumors.