Scanning Electrochemical Microscopy of Model Neurons: Imaging and Real-Time Detection of Morphological Changes
- 28 December 2002
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 75 (3), 563-571
- https://doi.org/10.1021/ac026166v
Abstract
Living PC12 cells, a model cell type for studying neuronal function, were imaged using the negative feedback mode of a scanning electrochemical microscope (SECM). Six biocompatible redox mediators were successfully identified from a large pool of candidates and were then used for imaging PC12 cells before and after exposure to nerve growth factor (NGF). When exposed to NGF, cells differentiate into a neuron phenotype by growing narrow neurites (1−2 μm wide) that can extend >100 μm from the cell proper. We demonstrate that carbon fiber electrodes with reduced tip diameters can be used for imaging both the cell proper and these neurites. Regions of decreased current, possibly resulting from raised features not identifiable by light microscopy, are clearly evident in the SECM images. Changes in the morphology of undifferentiated PC12 cells could be detected in real time with the SECM. After exposure to hypotonic and hypertonic solutions, reversible changes in cell height of <2 μm were measured.Keywords
This publication has 15 references indexed in Scilit:
- Scanning Electrochemical Microscopy (SECM): An Investigation of the Effects of Tip Geometry on Amperometric Tip ResponseThe Journal of Physical Chemistry B, 1998
- Imaging of Cellular Activity of Single Cultured Cells by Scanning Electrochemical MicroscopyChemistry Letters, 1998
- SPATIO-TEMPORAL RESOLUTION OF EXOCYTOSIS FROM INDIVIDUAL CELLSAnnual Review of Biophysics and Biophysical Chemistry, 1998
- Scanning electrochemical microscopy part 13. Evaluation of the tip shapes of nanometer size microelectrodesJournal of Electroanalytical Chemistry, 1992
- Characterization of submicron-sized carbon electrodes insulated with a phenol-allylphenol copolymerAnalytical Chemistry, 1992
- Aurintricarboxylic acid rescues PC12 cells and sympathetic neurons from cell death caused by nerve growth factor deprivation: correlation with suppression of endonuclease activity.The Journal of cell biology, 1991
- Bioaccumulation and measurement of copper at an alga-modified carbon paste electrodeAnalytical Chemistry, 1988
- The Nerve Growth Factor 35 Years LaterScience, 1987
- Bevelled carbon-fiber ultramicroelectrodesAnalytica Chimica Acta, 1986
- Mediator compounds for the electrochemical study of biological redox systems: a compilationAnalytica Chimica Acta, 1982