Tubular catabolism of albumin is associated with the release of an inflammatory lipid
- 30 June 1994
- journal article
- Published by Elsevier BV in Kidney International
- Vol. 45 (6), 1697-1709
- https://doi.org/10.1038/ki.1994.222
Abstract
Tubular catabolism of albumin is associated with the release of an inflammatory lipid. Proteinuria and tubulointerstitial inflammation (TII) correlate with progression to renal failure in human glomerulonephritis. Various forms of experimental nephrotic syndrome are associated with TII. To study the genesis of TII, we utilized the model of albumin overload. Rats received intraperitoneal bovine serum albumin (BSA) for 1 to 14 days, developing heavy proteinuria. A predominantly macrophage interstitial infiltrate was present at days 3, 7 and 14. The urine of the rats contained a factor chemotactic for macrophages which partitioned into the organic phase with ethyl acetate extraction. TLC and HPLC characteristics were those of a novel, non-polar lipid. Supernatant from the culture of proximal tubule (PT) segments after in vivo or in vitro exposure to high concentrations of lipid-replete BSA showed chemotactic activity with similar chromatographic characteristics. PT cultured with delipidated BSA produced little activity. Thus, the generation of this inflammatory factor occurs as a consequence of tubular metabolism of albumin-borne fatty acids and may contribute to the development of proteinuria-associated TIIKeywords
This publication has 33 references indexed in Scilit:
- Proteinuria and Progression in Human Glomerular DiseasesAmerican Journal of Nephrology, 1990
- T Cell recognition of epithelial selfAutoimmunity, 1989
- Expression of HLA-DQ, -DR, and -DP antigens in normal kidney and glomerulonephritisKidney International, 1989
- Significance of Tubulointerstitial Lesions in Biopsy Specimens of Glomerulonephritic PatientsAmerican Journal of Nephrology, 1989
- Synthesis of hydroxyeicosatetraenoic acids and leukotrienes in rat nephrotoxic serum glomerulonephritis. Role of anti-glomerular basement membrane antibody dose, complement, and neutrophiles.JCI Insight, 1988
- Energy thresholds that determine membrane integrity and injury in a renal epithelial cell line (LLC-PK1). Relationships to phospholipid degradation and unesterified fatty acid accumulation.JCI Insight, 1988
- The Relationship of Infiltrating Renal Leucocytes to Disease Activity in Lupus and Cryoglobulinaemic GlomerulonephritisNephron, 1988
- Acute tubulointerstitial nephritis associated with aminonucleoside nephrosisKidney International, 1988
- Glomerular podocytic injury in protein overload proteinuriaPathology, 1985
- MECHANISM OF PROTEINURIAThe Journal of Experimental Medicine, 1951