Infection induces antibodies against the cercarial secretions, but not against the cercarial elastases ofSchistosoma mansoni,Schistosoma haematobium,Schistosoma japonicumandTrichobilharzia ocellata
- 1 October 2001
- journal article
- research article
- Published by Wiley in Parasite Immunology
- Vol. 23 (10), 557-565
- https://doi.org/10.1046/j.1365-3024.2001.00417.x
Abstract
Cercarial secretions from different species of the parasite Schistosoma and from Trichobilharzia ocellata contain a proteolytic activity, cercarial elastase, which was demonstrated by a 30 kDa band in gelatin gels. Sera of patients infected with Schistosoma mansoni, Schistosoma haematobium or Schistosoma japonicum contain immunoglobulin G which react in ELISA with cercarial secretions from all schistosomes and cross-react among the different parasite species. In Western blots, however, infection sera from patients, as well as heavily infected mice or rabbits, did not react with a 30-kDa protein. Moreover, when sections from infected snails (Biomphalaria, Bulinus and Lymnaea) were analysed by immunofluorescence using the same infection sera, only the tegument of the developing cercariae was recognized, but not the acetabular glands. In contrast, when antisera against purified cercarial elastase from either S. mansoni or S. haematobium were tested with sections of infected Biomphalaria or Bulinus, fluorescence was strong in the preacetabular glands of the cercariae of either species, but undetectable with the tegument. Cross-reactivity of both antisera extended to T. ocellata-infected Lymnaea, but not to S. japonicum-infected Oncomelania. In conclusion, although immunization with purified cercarial elastase results in antibody production, the enzyme does not induce an apparent antibody response following natural infection.Keywords
This publication has 22 references indexed in Scilit:
- Schistosoma mansoni: anomalous immunogenic properties of a 27 kDa larval serine protease associated with protective immunityParasitology, 1997
- Complement regulation on the surface of cultured schistosomula and adult worms of Schistosoma mansoniEuropean Journal of Immunology, 1990
- Schistosome proteasesParasitology Today, 1988
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences of the United States of America, 1979
- Migration and development of Trichobilharzia ocellata (Trematoda: Schistosomatidae) in its duck hostsCanadian Journal of Zoology, 1973
- Schistosoma mansoni: Changes in the outer membrane of the tegument during development from cercaria to adult wormInternational Journal for Parasitology, 1973
- Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin GImmunochemistry, 1971
- Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin GMolecular Immunology, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Quantitative Studies of the Collagenase-like Enzymes of Cercariae of Schistosoma Mansoni and the Larvae of Strongyloides RatiiThe Journal of Infectious Diseases, 1956