Inaccuracy of Single-Target Sequencing for Discriminating Species of the Mycobacterium abscessus Group
- 1 August 2009
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 47 (8), 2596-2600
- https://doi.org/10.1128/jcm.00037-09
Abstract
We determined nucleotide sequences of rpoB , hsp65 , and sodA in 59 clinical isolates (from 58 patients) of the M ycobacterium abscessus group. Identification to the species level, based on three target genes, was concordant for 44 isolates (25 M. abscessus , 13 Mycobacterium massiliense , and 6 Mycobacterium bolletii isolates) and discordant for 15 isolates which had “interspecific composite patterns.” Sequence analysis of five housekeeping genes also showed composite patterns in 8 of these 15 isolates.Keywords
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