Assessing theIn VitroSuppressive Capacity of Regulatory T Cells
- 1 January 2007
- journal article
- review article
- Published by Taylor & Francis Ltd in Immunological Investigations
- Vol. 36 (5-6), 607-628
- https://doi.org/10.1080/08820130701790368
Abstract
Regulatory T cells (Treg) play a vital role in controlling peripheral immune responses in order to prevent autoimmunity and control inflammation. Altered Treg activities have been associated with the pathogenesis of multiple disorders including autoimmunity, allergy, cancer, and infection with persistent pathogens. As such, a great deal of interest has recently been directed towards developing additional tools and methods to better understand the mechanisms of suppression employed by Treg. The in vitro suppression assay has emerged as a valuable means by which to assess the functional capacity and activity of Treg. In this review, we summarize the merits and limitations of the various in vitro assays that have been utilized to assess Treg activity and present a novel two color proliferation assay that allows simultaneous monitoring of both regulatory and effector T cell activity. As further immunomodulatory therapies are explored, the need for additional methodologies to understand the cellular and molecular mechanisms of immune regulation conferred by Treg will play an increasingly important role.Keywords
This publication has 40 references indexed in Scilit:
- Adenosine generation catalyzed by CD39 and CD73 expressed on regulatory T cells mediates immune suppressionThe Journal of Experimental Medicine, 2007
- CD27 expression discriminates between regulatory and non‐regulatory cells after expansion of human peripheral blood CD4+ CD25+ cellsImmunology, 2007
- Expression of Interleukin-10 in Intestinal Lymphocytes Detected by an Interleukin-10 Reporter Knockin tiger MouseImmunity, 2006
- Imaging the function of regulatory T cells in vivoCurrent Opinion in Immunology, 2006
- Interleukin‐10‐secreting type 1 regulatory T cells in rodents and humansImmunological Reviews, 2006
- CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ T reg cellsThe Journal of Experimental Medicine, 2006
- Expression of interleukin (IL)-2 and IL-7 receptors discriminates between human regulatory and activated T cellsThe Journal of Experimental Medicine, 2006
- Visualizing regulatory T cell control of autoimmune responses in nonobese diabetic miceNature Immunology, 2005
- Rapamycin selectively expands CD4+CD25+FoxP3+ regulatory T cellsBlood, 2005
- Autoimmune Diseases as the Loss of Active “Self‐Control”Annals of the New York Academy of Sciences, 2003