Properties of a membrane-attached form of the folded chromosome of Escherichia coli
- 5 January 1974
- journal article
- Published by Elsevier BV in Journal of Molecular Biology
- Vol. 82 (1), 91-105
- https://doi.org/10.1016/0022-2836(74)90576-2
Abstract
Two types of DNA-containing particles are released from lysozyme-produced Escherichia coli spheroplasts after gentle lysis with non-ionic detergents in 1.-0 m-NaCl. Lysis at 25 °C releases the folded chromosomes (1300 S to 2200 S particles). Lysis at 10 °C results in faster sedimenting structures (3000 S to 4000 S). Both types of particles coexist in extracts of cells lysed at intermediate temperatures, i.e. 15 °C.Keywords
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