Correlation of the cytotoxic activity of four different alkaloids, from Chelidonium majus (greater celandine), with their DNA intercalating properties and ability to induce breaks in the DNA of NK/Ly murine lymphoma cells
Open Access
- 1 March 2006
- journal article
- Published by Walter de Gruyter GmbH in Open Life Sciences
- Vol. 1 (1), 2-15
- https://doi.org/10.2478/s11535-006-0001-y
Abstract
The purpose of this study was to examine the relationship between the DNA intercalating characteristics and the DNA damaging capacity of four alkaloids extracted from Chelidonium majus L, as well as their toxicity towards murine NK/Ly lymphoma cells. Chelerythrine, sanguinarine and coptisine were found to be intercalated into the DNA isolated from NK/Ly cells, meanwhile, chelidonine exhibited no affinity to DNA. Sanguinarine exhibited the greatest toxicity toward NK/Ly cells, and the toxicity of the other three decreased in descending order: chelerythrine, coptisine and chelidonine. Chelerythrine and sanguinarine caused DNA damage, illustrated by the formation of comets of the third class. Coptisine was less toxic than chelerythrine and sanguinarine, and affected the formation the same class of comets in higher concentration. The quantity of comets induced by chelidonine were negligible, a finding consistent with its inability to intercalate into DNA structure. The ability of four main alkaloids of Chelidonium majus L., to intercalate into DNA isolated from murine NK/Ly lymphoma cells, correlated with their ability to induce breaks in cellular DNA and with their toxic effect towards those cells.Keywords
This publication has 19 references indexed in Scilit:
- Processing of clustered DNA damage generates additional double-strand breaks in mammalian cells post-irradiationNucleic Acids Research, 2004
- Inhibition of mouse liver respiration by Chelidonium majus isoquinoline alkaloidsToxicology Letters, 2003
- Death by necrosisEMBO Reports, 2002
- Bax, Bcl-2, and NF-κB Expression in Sanguinarine Induced Bimodal Cell DeathExperimental and Molecular Pathology, 2001
- The effects of chelidonine on tubulin polymerisation, cell cycle progression and selected signal transmission pathwaysEuropean Journal of Cell Biology, 2001
- PHARMACOLOGICAL ACTIVITIES OFCHELIDONIUM MAJUSL. (PAPAVERACEAE)Pharmacological Research, 1996
- Binding of sanguinarine to deoxyribonucleic acids of differing base compositionBiochemical Pharmacology, 1985
- Antiinflammatory Activity of Quaternary Benzophenanthridine Alkaloids fromChelidonoum majus*,**Planta Medica, 1981
- Minor alkaloids from Chelidonium majus L.Collection of Czechoslovak Chemical Communications, 1977
- Alkaloide der Mohngewächse (Papaveraceae) XXX. Über weitere Alkaloide aus der Wurzel von Chelidonium majus L.Collection of Czechoslovak Chemical Communications, 1965