Identification and Characterization of Pathogenic Aeromonas veronii Biovar Sobria Associated with Epizootic Ulcerative Syndrome in Fish in Bangladesh
Open Access
- 1 February 2002
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 68 (2), 650-655
- https://doi.org/10.1128/aem.68.2.650-655.2002
Abstract
Sparse information is available on the virulence factors of Aeromonas strains isolated from diseased fish, from the environment, and from humans. In the present study, 52 Aeromonas isolates obtained from epizootic ulcerative syndrome (EUS) lesions in fish, from the aquatic environment, and from children with diarrhea in Bangladesh were identified by biochemical phenotyping (i.e., PhenePlate [PhP] typing) and DNA fingerprinting and then characterized with respect to certain putative virulence factors. The isolates from the fish exhibiting EUS symptoms were identified to be Aeromonas veronii biovar sobria by fatty acid methyl ester analysis and amplified fragment length polymorphism fingerprinting. Biochemical phenotyping revealed that all EUS-associated isolates belonged to a unique phenotype which was not identified among more than 1,600 environmental and diarrheal isolates in a previously collected database of PhP types of Bangladeshi Aeromonas isolates. The 52 Aeromonas isolates were investigated for the production of hemolysin and cytotoxin; for hemagglutination with erythrocytes from fish, human, and rabbit sources; for the presence of a cytolytic enterotoxin gene; and for adhesion to and invasion into fish cell lines. All of the EUS isolates produced all of the virulence factors investigated, as did also some of the environmental isolates, but the isolates from EUS were unique in their ability to agglutinate fish erythrocytes. Our results suggest that a clonal group of A. veronii biovar sobria is associated with, and may be a causative agent of, EUS in fish in Bangladesh.Keywords
This publication has 22 references indexed in Scilit:
- Evaluation of a fluorescent amplified fragment length polymorphism (FAFLP) methodology for the genotypic discrimination ofAeromonastaxaFEMS Microbiology Letters, 1999
- The role of flagella and motility in the adherence and invasion to fish cell lines by Aeromonas hydrophila serogroup O:34 strainsFEMS Microbiology Letters, 1997
- Adhesion ofAeromonassp. to cell lines used as models for intestinal adhesionEpidemiology and Infection, 1995
- Diversity of Aeromonas sp. in Flemish drinking water production plants as determined by gas‐liquid chromatographic analysis of cellular fatty acid methyl esters (FAMEs)Journal of Applied Bacteriology, 1995
- Cellular Fatty Acid Composition as a Chemotaxonomic Marker for the Differentiation of Phenospecies and Hybridization Groups in the Genus AeromonasInternational Journal of Systematic and Evolutionary Microbiology, 1994
- Distribution of Aeromonas hydrophila hybridization groups and their virulence properties in Australasian clinical and environmental strainsLetters in Applied Microbiology, 1994
- The pathogenicity of Aeromonas strains relative to genospecies and phenospecies identificationFEMS Microbiology Letters, 1991
- A SIMPLE AND EXACT TWO‐POINT INTERPOLATION METHOD FOR DETERMINATION OF HAEMOLYTIC ACTIVITY IN MICROTITER PLATESActa Pathologica Microbiologica Scandinavica Series B: Microbiology, 1987
- Adhesion ofAeromonas hydrophilaandVibrio anguillarumto fish cells and to mucus-coated glass slidesFEMS Microbiology Letters, 1987
- Adhesion ofEscherichia colito human periurethral cells correlated to mannose-resistant agglutination of human erythrocytesFEMS Microbiology Letters, 1979