A simple gravimetric technique for measurement of cerebral edema

Abstract

A simple method was developed for the laboratory preparation of gradient columns of specific gravity used in measurement of brain-tissue water. By this automated technique, virtually linear and repeatable density gradients were obtained from which values of tissue specific gravity could be determined. The specific gravity of both solid and fresh cortex and white matter from adult cats was measured and converted to units of percent H2O per gram tissue using conversion factors derived for this purpose and applicable to studies of brain edema.