An astrovirus frameshift signal induces ribosomal frameshifting in vitro
- 1 June 1995
- journal article
- Published by Springer Science and Business Media LLC in Archiv für die gesamte Virusforschung
- Vol. 140 (6), 1127-1135
- https://doi.org/10.1007/bf01315421
Abstract
Expression of the astrovirus RNA-dependent RNA polymerase has been hypothesized to be regulated by (− 1) ribosomal frameshifting. Sequence analysis of the 70 nucleotide region between open reading frames 1a and 1b indicates the presence of a shifty heptamer consensus sequence and downstream sequences that may be needed for ribosomal frameshifting. We constructed four astrovirus cassettes that spanned this region and inserted each into the rhesus rotavirus VP4 gene. The constructs were expressed in an in vitro system, and products were immunoprecipitated by rotavirus amino and carboxy terminal-specific monoclonal antibodies. Ribosomal frameshifting, at an efficiency of 6–7%, was demonstrated in all constructs containing the shifty heptamer and stem-loop. Deletion of the downstream sequence potentially involved in pseudoknot formation did not affect frameshifting efficiency. However, deletion of the shifty heptamer resulted in no detectable frameshift activity.Keywords
This publication has 24 references indexed in Scilit:
- The complete sequence of a human astrovirusJournal of General Virology, 1994
- Prevalence of human astrovirus serotypes in the Oxford region 1976–92, with evidence for two new serotypesEpidemiology and Infection, 1994
- Astroviruses as a Cause of Gastroenteritis in ChildrenThe New England Journal of Medicine, 1991
- Structure and Function of Suppressor tRNAs in Higher EukaryoteCritical Reviews in Biochemistry and Molecular Biology, 1990
- Translational Suppression in Gene Expression in Retroviruses and RetrotransposonsCurrent Topics in Microbiology and Immunology, 1990
- Signals for ribosomal frameshifting in the rous sarcoma virus gag-pol regionCell, 1988
- Expression of the Rous Sarcoma Virus pol Gene by Ribosomal FrameshiftingScience, 1985
- HUMAN ASTROVIRUS SEROTYPESThe Lancet, 1984
- Serial Propagation of Astrovirus in Tissue Culture with the Aid of TrypsinJournal of General Virology, 1981
- VIRUSES IN INFANTILE GASTROENTERITISThe Lancet, 1975