Imaging inflammation of the pancreatic islets in type 1 diabetes
Open Access
- 10 August 2004
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 101 (34), 12634-12639
- https://doi.org/10.1073/pnas.0404307101
Abstract
Type 1 diabetes is the clinical manifestation of aberrant leukocytic infiltration of the pancreatic islets; it is usually diagnosed only very late in disease progression, after the critical autoimmune phenomena have mostly played out. A noninvasive means of directly monitoring the evolution of islet infiltrates would have important research and clinical applications. We have exploited fluorescence and MRI of long-circulating magnetofluorescent nanoparticles to visualize micro-vascular leakage, as an indicator of inflammation, in pancreata of mouse models of type 1 diabetes ex vivo or in vivo. We could detect the onset and evolution of insulitis in vivo and in real time, permitting us to study the natural history of diabetes in individual animals.This publication has 30 references indexed in Scilit:
- Water-Soluble Quantum Dots for Multiphoton Fluorescence Imaging in VivoScience, 2003
- Sequential conversion of the redox status of macrophages dictates the pathological progression of autoimmune diabetesEuropean Journal of Immunology, 2003
- Th1 and Th2 cytokines exert regulatory effects upon islet microvascular areas in the NOD mouseJournal of Cellular Biochemistry, 2002
- Subsets of Macrophages and Dendritic Cells in Nonobese Diabetic Mouse Pancreatic Inflammatory Infiltrates: Correlation with the Development of DiabetesLaboratory Investigation, 2000
- Approaches and agents for imaging the vascular systemAdvanced Drug Delivery Reviews, 1999
- High-Efficiency Intracellular Magnetic Labeling with Novel Superparamagnetic-Tat Peptide ConjugatesBioconjugate Chemistry, 1999
- Pancreatic Islet Blood Perfusion in the Nonobese Diabetic Mouse: Diabetes-Prone Female Mice Exhibit a Higher Blood Flow Compared with Male Mice in the Prediabetic PhaseEndocrinology, 1998
- Following a diabetogenic T cell from genesis through pathogenesisCell, 1993
- THE ROLE OF ENDOTHELIAL CELLS IN INFLAMMATIONTransplantation, 1990
- MHC-Linked Protection from Diabetes Dissociated from Clonal Deletion of T CellsScience, 1990