The ubiquitin ligase HACE1 regulates Golgi membrane dynamics during the cell cycle
- 1 September 2011
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Communications
- Vol. 2 (1), 1-11
- https://doi.org/10.1038/ncomms1509
Abstract
Partitioning of the Golgi membrane into daughter cells during mammalian cell division occurs through a unique disassembly and reassembly process that is regulated by ubiquitination. However, the identity of the ubiquitin ligase is unknown. Here we show that the Homologous to the E6-AP Carboxyl Terminus (HECT) domain containing ubiquitin ligase HACE1 is targeted to the Golgi membrane through interactions with Rab proteins. The ubiquitin ligase activity of HACE1 in mitotic Golgi disassembly is required for subsequent postmitotic Golgi membrane fusion. Depletion of HACE1 using small interfering RNAs or expression of an inactive HACE1 mutant protein in cells impaired postmitotic Golgi membrane fusion. The identification of HACE1 as a Golgi-localized ubiquitin ligase provides evidence that ubiquitin has a critical role in Golgi biogenesis during the cell cycle.Keywords
This publication has 45 references indexed in Scilit:
- Reconstitution of the cell cycle-regulated Golgi disassembly and reassembly in a cell-free systemNature Protocols, 2010
- Quantitative Proteomics Analysis of Cell Cycle-regulated Golgi Disassembly and ReassemblyOnline Journal of Public Health Informatics, 2010
- The Role of GRASP65 in Golgi Cisternal Stacking and Cell Cycle ProgressionTraffic, 2010
- GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stackingThe Journal of cell biology, 2010
- Molecular Mechanism of Mitotic Golgi Disassembly and Reassembly Revealed by a Defined Reconstitution AssayOnline Journal of Public Health Informatics, 2008
- Active ADP-ribosylation Factor-1 (ARF1) Is Required for Mitotic Golgi FragmentationOnline Journal of Public Health Informatics, 2007
- Mapping the Functional Domains of the Golgi Stacking Factor GRASP65Journal of Biological Chemistry, 2005
- GS15 Forms a SNARE Complex with Syntaxin 5, GS28, and Ykt6 and Is Implicated in Traffic in the Early Cisternae of the Golgi ApparatusMolecular Biology of the Cell, 2002
- Sequential SNARE disassembly and GATE-16–GOS-28 complex assembly mediated by distinct NSF activities drives Golgi membrane fusionThe Journal of cell biology, 2002
- Purification and Identification of Novel Rab Effectors Using Affinity ChromatographyMethods, 2000