Association of single nucleotide polymorphisms in vascular endothelial growth factor gene with bladder cancer risk
- 21 February 2013
- journal article
- Published by Springer Science and Business Media LLC in Medical Oncology
- Vol. 30 (2), 1-7
- https://doi.org/10.1007/s12032-013-0509-8
Abstract
Vascular endothelial growth factor (VEGF) is an endothelial cell-specific potent mitogen involved in a number of pathologic processes, including angiogenesis, tumor growth, and metastasis. Polymorphisms of VEGF gene have been associated with susceptibility to several cancers. VEGF gene susceptibility to bladder cancer (BC), however, still remains controversial. We analyzed association of the -2578C/A, -7C/T, -2549I/D, and -1001G/C polymorphism of the VEGF gene with bladder cancer (BC) risk and interaction of these polymorphisms with lifestyle and demographic factors. We genotyped -2578C/A, -7C/T, -2549I/D and -1001G/C by PCR–restriction fragment length polymorphism and amplification refractory mutation specific method to evaluate risk in 200 BC patients and 250 healthy controls from North Indian population. Significant association for BC risk in heterozygous CA genotype (1.69-fold) in VEGF-2578C/A and heterozygous genotype of VEGF-1001G/C (p = 0.032) was observed. Interestingly, VEGF-2549I/D genotype showed reduced risk for BC. The gene–gene combination analysis revealed DD-GG with reduced risk (p = 0.018) of VEGF-2549I/D and VEGF-1001G/C, and combination CA-GG of VEGF-2578C/A and VEGF-1001G/C demonstrated 1.75-fold risk for BC. Our findings suggested that polymorphism -2578C/A and -1001G/C in the promoter of VEGF gene may play a significant role in mediating the bladder cancer risk, whereas VEGF-2549I/D genotype appears to be protective for BC.Keywords
This publication has 21 references indexed in Scilit:
- Association of Common Variants of Vascular Endothelial Growth Factor and Interleukin-18 Genes with Allograft Survival in Renal Transplant Recipients of North IndiaDNA and Cell Biology, 2011
- Vascular endothelial growth factor gene polymorphisms in North Indian patients with recurrent miscarriagesReproductive BioMedicine Online, 2011
- Polymorphisms in the vascular endothelial growth factor gene and the risk of familial endometriosisMolecular Human Reproduction, 2008
- Pharmacogenetics of EGFR and VEGF inhibitionDrug Discovery Today, 2007
- Association of the VEGF gene polymorphism with diabetic retinopathy in type 2 diabetes patientsNephrology Dialysis Transplantation, 2007
- Antivascular Endothelial Growth Factor Agents and Their Development: Therapeutic Implications in Ocular DiseasesAmerican Journal of Ophthalmology, 2006
- VEGF-A stimulation of leukocyte adhesion to colonic microvascular endothelium: implications for inflammatory bowel diseaseAmerican Journal of Physiology-Gastrointestinal and Liver Physiology, 2006
- Shear stress and VEGF activate IKK via the Flk-1/Cbl/Akt signaling pathwayAmerican Journal of Physiology-Heart and Circulatory Physiology, 2004
- IDENTIFICATION OF POLYMORPHISMS WITHIN THE VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) GENE: CORRELATION WITH VARIATION IN VEGF PROTEIN PRODUCTIONCytokine, 2000
- VASCULAR ENDOTHELIAL GROWTH FACTOR AS PROGNOSTIC FACTOR IN RENAL CELL CARCINOMAJournal of Urology, 2000