Organelle segregation into Plasmodium liver stage merozoites
Open Access
- 29 July 2011
- journal article
- Published by Hindawi Limited in Cellular Microbiology
- Vol. 13 (11), 1768-1782
- https://doi.org/10.1111/j.1462-5822.2011.01657.x
Abstract
The liver stage of the Plasmodium parasite remains one of the most promising targets for intervention against malaria as it is clinically silent, precedes the symptomatic blood stage and represents a bottleneck in the parasite life cycle. However, many aspects of the development of the parasite during this stage are far from understood. During the liver stage, the parasite undergoes extensive replication, forming tens of thousands of infectious merozoites from each invading sporozoite. This implies a very efficient and accurate process of cytokinesis and thus also of organelle development and segregation. We have generated for the first time Plasmodium berghei double-fluorescent parasite lines, allowing visualization of the apicoplast, mitochondria and nuclei in live liver stage parasites. Using these we have seen that in parallel with nuclear division, the apicoplast and mitochondrion become two extensively branched and intertwining structures. The organelles then undergo impressive morphological and positional changes prior to cell division. To form merozoites, the parasite undergoes cytokinesis and the complex process of organelle development and segregation into the forming daughter merozoites could be analysed in detail using the newly generated transgenic parasites.Keywords
This publication has 23 references indexed in Scilit:
- Identification and Characterization of a Liver Stage-Specific Promoter Region of the Malaria Parasite PlasmodiumPLOS ONE, 2010
- Positioning of large organelles by a membrane- associated cytoskeleton inPlasmodiumsporozoitesCellular Microbiology, 2010
- Going live: A comparative analysis of the suitability of the RFP derivatives RedStar, mCherry and tdTomato for intravital andin vitrolive imaging ofPlasmodiumparasitesBiotechnology Journal, 2009
- Organellar dynamics during the cell cycle of Toxoplasma gondiiJournal of Cell Science, 2008
- Metabolic maps and functions of thePlasmodiummitochondrionFEMS Microbiology Reviews, 2006
- High-efficiency transfection and drug selection of genetically transformed blood stages of the rodent malaria parasite Plasmodium bergheiNature Protocols, 2006
- Quantitative imaging of Plasmodium transmission from mosquito to mammalNature Medicine, 2006
- Development of the endoplasmic reticulum, mitochondrion and apicoplast during the asexual life cycle of Plasmodium falciparumMolecular Microbiology, 2005
- The Plastid in Plasmodium falciparum Asexual Blood Stages: a Three-Dimensional Ultrastructural AnalysisProtist, 1999
- Transformation of sporozoites of Plasmodium berghei into exoerythrocytic forms in the liver of its mammalian hostCell and tissue research, 1985