Mass spectrometry-based proteomics: existing capabilities and future directions
- 13 April 2012
- journal article
- review article
- Published by Royal Society of Chemistry (RSC) in Chemical Society Reviews
- Vol. 41 (10), 3912-3928
- https://doi.org/10.1039/c2cs15331a
Abstract
Mass spectrometry (MS)-based proteomics is emerging as a broadly effective means for identification, characterization, and quantification of proteins that are integral components of the processes essential for life. Characterization of proteins at the proteome and sub-proteome (e.g., the phosphoproteome, proteoglycome, or degradome/peptidome) levels provides a foundation for understanding fundamental aspects of biology. Emerging technologies such as ion mobility separations coupled with MS and microchip-based-proteome measurements combined with MS instrumentation and chromatographic separation techniques, such as nanoscale reversed phase liquid chromatography and capillary electrophoresis, show great promise for both broad undirected and targeted highly sensitive measurements. MS-based proteomics increasingly contribute to our understanding of the dynamics, interactions, and roles that proteins and peptides play, advancing our understanding of biology on a systems wide level for a wide range of applications including investigations of microbial communities, bioremediation, and human health.Keywords
This publication has 92 references indexed in Scilit:
- Target-Decoy Approach and False Discovery Rate: When Things May Go WrongJournal of the American Society for Mass Spectrometry, 2011
- Improving Liquid Chromatography-Mass Spectrometry Sensitivity Using a Subambient Pressure Ionization with Nanoelectrospray (SPIN) InterfaceJournal of the American Society for Mass Spectrometry, 2011
- Reversed‐phase chromatography with multiple fraction concatenation strategy for proteome profiling of human MCF10A cellsProteomics, 2011
- Nuclear protein isoforms: Implications for cancer diagnosis and therapyJournal of Cellular Biochemistry, 2010
- Two-dimensional liquid chromatography system for online top-down mass spectrometryProteomics, 2010
- FT-ICR MS optimization for the analysis of intact proteinsInternational Journal of Mass Spectrometry, 2009
- Dilution-Free Analysis from Picoliter Droplets by Nano-Electrospray Ionization Mass SpectrometryAngewandte Chemie-International Edition, 2009
- Full Dynamic Range Proteome Analysis of S. cerevisiae by Targeted ProteomicsCell, 2009
- The ion funnel: Theory, implementations, and applicationsMass Spectrometry Reviews, 2009
- Advances in proteomics data analysis and display using an accurate mass and time tag approachMass Spectrometry Reviews, 2006