Reversibility of metabolic and morphological changes associated with chronic exposure of pancreatic islet β‐cells to fatty acids
- 12 January 2010
- journal article
- research article
- Published by Wiley in Journal of Cellular Biochemistry
- Vol. 109 (4), 683-692
- https://doi.org/10.1002/jcb.22445
Abstract
Pancreatic β-cells metabolise both lipid and glucose nutrients but chronic exposure (>24 h) to elevated fatty acid (FA) concentrations results in deleterious metabolic and morphological changes. The aims of this study were to assess the adaptive morphological, metabolic and secretory responses of islet β-cells to exposure and removal of FA. Isolated mouse islets and INS-1 β-cells were exposed to oleate or palmitate (0.5 mM) or a 1:1 mixture of both FA for 48 h prior to a 24 h period without FA. Subsequent changes in lipid storage and composition (triglycerides, TG and phospholipids, PL), gene expression, β-cell morphology and glucose-stimulated insulin secretion (GSIS) were determined. Intracellular TG content increased during exposure to FA and was lower in cells subsequently incubated in FA-free media (P < 0.05); TG storage was visible as oil red O positive droplets (oleate) by light microscopy or ‘splits’ (palmitate) by electron microscopy. Significant desaturation of β-cell FA occurred after exposure to oleate and palmitate. After incubation in FA-free media, there was differential handling of specific FA in TG, resulting in a profile that tended to revert to that of control cells. FA treatment resulted in elevated lipolysis of intracellular TG, increased FA oxidation and reduced GSIS. After incubation in FA-free media, oxidation remained elevated but inhibition of FA oxidation with etomoxir (10 µM) had no effect on the improvement in GSIS. The β-cell demonstrates metabolic flexibility as an adaptive response to ambient concentrations of FA. J. Cell. Biochem. 109: 683–692, 2010.Keywords
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