Near-infrared narrow-band imaging of gold/silica nanoshells in tumors
- 1 January 2009
- journal article
- Published by SPIE-Intl Soc Optical Eng in Journal of Biomedical Optics
- Vol. 14 (2), 024044
- https://doi.org/10.1117/1.3120494
Abstract
Gold nanoshells (GNS) are a new class of nanoparticles that can be optically tuned to scatter or absorb light from the near-ultraviolet to near-infrared (NIR) region by varying the core (dielectric silica)/shell (gold) ratio. In addition to spectral tunability, GNS are inert and bioconjugatable, making them potential labels for in vivo imaging and therapy of tumors. We report the use of GNS as exogenous contrast agents for enhanced visualization of tumors using narrow-band imaging (NBI). NBI takes advantage of the strong NIR absorption of GNS to distinguish between blood and nanoshells in the tumor by imaging in narrow wavelength bands in the visible and NIR, respectively. Using tissue-simulating phantoms, we determined the optimum wavelengths to enhance contrast between blood and GNS. We then used the optimum wavelengths for ex vivo imaging of tumors extracted from human colon cancer xenograft bearing mice injected with GNS. Systemically delivered GNS accumulated passively in tumor xenografts by the enhanced permeability and retention (EPR) effect. Ex vivo NBI of tumor xenografts demonstrated heterogeneous distribution of GNS with a clear distinction from the tumor vasculature. The results of this study demonstrate the feasibility of using GNS as contrast agents to visualize tumors using NBI.Keywords
This publication has 24 references indexed in Scilit:
- Enhanced multi-spectral imaging of live breast cancer cells using immunotargeted gold nanoshells and two-photon excitation microscopyNanotechnology, 2008
- Near-Infrared Resonant Nanoshells for Combined Optical Imaging and Photothermal Cancer TherapyNano Letters, 2007
- In Vivo Detection of Gold Nanoshells in Tumors Using Diffuse Optical SpectroscopyIEEE Journal of Selected Topics in Quantum Electronics, 2007
- Quantitative evaluation of optical coherence tomography signal enhancement with gold nanoshellsJournal of Biomedical Optics, 2006
- Selective laser photo-thermal therapy of epithelial carcinoma using anti-EGFR antibody conjugated gold nanoparticlesCancer Letters, 2005
- Immunotargeted Nanoshells for Integrated Cancer Imaging and TherapyNano Letters, 2005
- Narrow-Band Imaging in the Diagnosis of Colorectal Mucosal Lesions: A Pilot StudyEndoscopy, 2004
- Photoacoustic Tomography of a Nanoshell Contrast Agent in the in Vivo Rat BrainNano Letters, 2004
- Photo-thermal tumor ablation in mice using near infrared-absorbing nanoparticlesCancer Letters, 2004
- Appearance of enhanced tissue features in narrow-band endoscopic imagingJournal of Biomedical Optics, 2004