Whole cigarette smoke promotes human gingival epithelial cell apoptosis and inhibits cell repair processes
- 25 April 2011
- journal article
- Published by Wiley in Journal of Periodontal Research
- Vol. 46 (5), 533-541
- https://doi.org/10.1111/j.1600-0765.2011.01370.x
Abstract
Smoking cigarettes increases the risk of developing various types of human diseases, including cancers and periodontitis. As gingival epithelial cells are known to play an active role in innate immunity via the secretion of a wide variety of mediators, and as these cells are the first ones exposed to environmental stimuli such as cigarette smoke, we sought to investigate the effects of whole cigarette smoke on normal human gingival epithelial cells and tissue. Human gingival epithelial cells were extracted from healthy nonsmokers and used either as a monolayer or as an engineered human oral mucosa to investigate the effect of whole cigarette smoke on cell growth, apoptosis and wound repair/migration. Our findings show that when gingival epithelial cells were exposed once to whole cigarette smoke, this resulted in a significant inhibition of cell growth through an apoptotic pathway, as confirmed by an increase of Bax and a decrease of Bcl-xL and caspase-3 activity. Cigarette smoke also inhibited epithelial cell migration. These effects may explain the disorganization of the engineered human oral mucosa tissue when exposed to whole cigarette smoke. Exposure to whole cigarette smoke markedly inhibits epithelial cell growth through an apoptosis/necrosis pathway that involves Bax and Bcl-xL proteins and caspase-3 activity. Cigarette smoke also disrupts epithelial cell migration, which may negatively affect periodontal wound healing.Keywords
This publication has 45 references indexed in Scilit:
- Sex Differences in Destructive Periodontal Disease: Exploring the Biologic BasisThe Journal of Periodontology, 2010
- Free Gingival Grafts: Graft Shrinkage and Donor‐Site Healing in Smokers and Non‐SmokersThe Journal of Periodontology, 2010
- Regenerative treatment with platelet‐rich plasma combined with a bovine‐derived xenograft in smokers and non‐smokers: 12‐month clinical and radiographic resultsJournal of Clinical Periodontology, 2009
- Effects of smoking on healing response to non‐surgical periodontal therapy: a multilevel modelling analysisJournal of Clinical Periodontology, 2009
- The impact of cigarette smoking on periodontal disease and treatmentPeriodontology 2000, 2007
- Longitudinal Study of the Association Between Smoking as a Periodontitis Risk and Salivary Biomarkers Related to PeriodontitisThe Journal of Periodontology, 2007
- Gingival Recession Treatment With Connective Tissue Grafts in Smokers and Non‐SmokersThe Journal of Periodontology, 2006
- The Influence of Tobacco Smoking on the Onset of Periodontitis in Young PersonsTobacco Induced Diseases, 2004
- Effects of smoking on periodontal tissuesJournal of Clinical Periodontology, 2002
- ALVEOLAR BONE LOSS AND TOOTH LOSS IN MALE CIGAR AND PIPE SMOKERSThe Journal of the American Dental Association, 1999