Comparative Analysis of Serum (Anti)oxidative Status Parаmeters in Healthy Persons
Open Access
- 17 March 2013
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 14 (3), 6106-6115
- https://doi.org/10.3390/ijms14036106
Abstract
Five antioxidant and two oxidative stress assays were applied to serum samples of 43 healthy males. The antioxidant tests showed different inter-assay correlations. A very good correlation of 0.807 was observed between the ferric reducing ability of plasma (FRAP) and total antioxidant status (TAS) assay and also a fair correlation of 0.501 between the biological antioxidant potential (BAP) and TAS assay. There was no statistically significant correlation between the BAP and FRAP assay. The anti-oxidant assays have a high correlation with uric acid, especially the TAS (0.922) and FRAP assay (0.869). The BAP assay has a much lower and no statistically significant correlation with uric acid (0.302), which makes BAP more suitable for the antioxidant status. The total thiol assay showed no statistically significant correlation with uric acid (0.114). The total thiol assay, which is based on a completely different principle, showed a good and statistically significant correlation with the BAP assay (0.510) and also to the TAS assay, but to a lower and not significant extent (0.279) and not with the FRAP assay (−0.008). The oxy-adsorbent test (OXY) assay has no correlation with any of the other assays tested. The oxidative stress assays, reactive oxygen metabolites (ROM) and total oxidant status (TOS), based on a different principle, do not show a statistically significant correlation with the serum samples in this study. Both assays showed a negative, but not significant, correlation with the antioxidant assays. In conclusion, the ROM, TOS, BAP and TTP assays are based on different principles and will have an additional value when a combination of these assays will be applied in large-scale population studies.Keywords
This publication has 32 references indexed in Scilit:
- Biomarkers of Oxidative Stress and Risk of Developing Colorectal Cancer: A Cohort-nested Case-Control Study in the European Prospective Investigation Into Cancer and NutritionAmerican Journal of Epidemiology, 2012
- The Role of Oxidative Stress on the Pathogenesis of Graves' DiseaseJournal of Thyroid Research, 2011
- The Effects of a Multiflavonoid Supplement on Vascular and Hemodynamic Parameters following Acute ExerciseOxidative Medicine and Cellular Longevity, 2011
- Oxidative Stress in Neurodegenerative Diseases: Mechanisms and Therapeutic PerspectivesOxidative Medicine and Cellular Longevity, 2011
- Plasma antioxidant enzymes and clastogenic factors as possible biomarkers of colorectal cancer riskMutation Research, 2011
- Evaluation of Oxidative Stress in Type 2 Diabetes Mellitus and Follow-up Along with Vitamin E SupplementationIndian Journal of Clinical Biochemistry, 2010
- HDL metabolism and activity in chronic kidney diseaseNature Reviews Nephrology, 2010
- Downregulation of Adipose Glutathione S-Transferase A4 Leads to Increased Protein Carbonylation, Oxidative Stress, and Mitochondrial DysfunctionDiabetes, 2010
- Reduction of serum antioxidative capacity during hemodialysisClinical and Experimental Nephrology, 2007
- Endothelial progenitor cells for postnatal vasculogenesisClinical and Experimental Nephrology, 2007