Identification of a Gene Associated with Bt Resistance in Heliothis virescens
Top Cited Papers
- 3 August 2001
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 293 (5531), 857-860
- https://doi.org/10.1126/science.1060949
Abstract
Transgenic crops producing insecticidal toxins from Bacillus thuringiensis (Bt) are widely used for pest control. Bt-resistant insect strains have been studied, but the molecular basis of resistance has remained elusive. Here, we show that disruption of a cadherin-superfamily gene by retrotransposon-mediated insertion was linked to high levels of resistance to the Bt toxin Cry1Ac in the cotton pest Heliothis virescens. Monitoring the early phases of Bt resistance evolution in the field has been viewed as crucial but extremely difficult, especially when resistance is recessive. Our findings enable efficient DNA-based screening for resistant heterozygotes by directly detecting the recessive allele.Keywords
This publication has 15 references indexed in Scilit:
- The cadherin‐like protein is essential to specificity determination and cytotoxic action of the Bacillus thuringiensis insecticidal CryIAa toxinFEBS Letters, 1999
- The Heliothis virescens 170kDa aminopeptidase functions as “Receptor A” by mediating specific Bacillus thuringiensis Cry1A δ-endotoxin binding and pore formationInsect Biochemistry and Molecular Biology, 1997
- Identification of a Linkage Group with a Major Effect on Resistance to Bacillus thuringiensis CrylAc Endotoxin in the Tobacco Budworm (Lepidoptera: Noctuidae)Journal of Economic Entomology, 1997
- Selection and Genetic Analysis of a Heliothis virescens (Lepidoptera: Noctuidae) Strain with High Levels of Resistance to Bacillus thuringiensis ToxinsJournal of Economic Entomology, 1995
- Identification, Isolation, and Cloning of a Bacillus thuringiensis CryIAc Toxin-binding Protein from the Midgut of the Lepidopteran Insect Heliothis virescensPublished by Elsevier BV ,1995
- Cloning and Expression of a Receptor for an Insecticidal Toxin of Bacillus thuringiensisPublished by Elsevier BV ,1995
- Resistance to the Bacillus thuringiensis bioinsecticide in a field population of Plutella xylostella is due to a change in a midgut membrane receptor.Proceedings of the National Academy of Sciences of the United States of America, 1991
- Mechanism of Insect Resistance to the Microbial Insecticide Bacillus thuringiensisScience, 1990
- Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphismsNature, 1988
- MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populationsGenomics, 1987