Iron-Hematoxylin and Safranin O As A Polychrome Stain for Epon Sections
- 1 January 1965
- journal article
- research article
- Published by Informa UK Limited in Stain Technology
- Vol. 40 (5), 279-282
- https://doi.org/10.3109/10520296509116427
Abstract
A 3-step procedure performed at 85 C was as follows: Sections were treated with 4% aqueous ferric ammonium sulfate for 15-300 sec, washed, placed in ripened, 0.5% hematoxylin for 15-300 sec, washed, and counterstained in 1% Safranin O for 15-90 sec. This procedure produced a durable stain with clear cytoplasmic and nuclear differentiation.Keywords
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