The Single-Probe: A Miniaturized Multifunctional Device for Single Cell Mass Spectrometry Analysis
- 15 September 2014
- journal article
- editorial
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 86 (19), 9376-9380
- https://doi.org/10.1021/ac5029038
Abstract
We have developed a new mass spectrometry (MS) technology, the Single-probe MS, capable of real-time, in situ metabolomic analysis of individual living cells. The Single-probe is a miniaturized multifunctional sampling and ionization device that is directly coupled to the mass spectrometer. With a sampling tip smaller than individual eukaryotic cells (<10 μm), the Single-probe can be inserted into single cells to sample the intracellular compounds for real-time MS analysis. We have used the Single-probe to detect several cellular metabolites and the anticancer small molecules paclitaxel, doxorubicin, and OSW-1 in individual cervical cancer cells (HeLa). Single cell mass spectrometry (SCMS) is an emerging scientific technology that could reshape the analytical science of many research disciplines, and the Single-probe MS technology is a novel method for SCMS that, through its accessible fabrication protocols, can be broadly applied to different research areas.Keywords
This publication has 39 references indexed in Scilit:
- Highly multiplexed quantitation of gene expression on single cellsJournal of Immunological Methods, 2013
- Single Cell Profiling of Circulating Tumor Cells: Transcriptional Heterogeneity and Diversity from Breast Cancer Cell LinesPLOS ONE, 2012
- Comprehensive qPCR profiling of gene expression in single neuronal cellsNature Protocols, 2011
- Profiling metabolites and peptides in single cellsNature Methods, 2011
- Single cell transcriptional profiling reveals heterogeneity of human induced pluripotent stem cellsJCI Insight, 2011
- Single cell analysis: the new frontier in ‘omics’Trends in Biotechnology, 2010
- Mass Spectrometric Imaging for Biomedical Tissue AnalysisChemical Reviews, 2010
- The Origin of Phenotypic Heterogeneity in a Clonal Cell Population In VitroPLOS ONE, 2007
- Microtechnologies and nanotechnologies for single-cell analysisCurrent Opinion in Biotechnology, 2004
- Imaging with Mass SpectrometryChemical Reviews, 1999