Detection ofTreponema pallidumin Aqueous Humor by Real-time Polymerase Chain Reaction
- 23 March 2011
- journal article
- research article
- Published by Taylor & Francis Ltd in Ocular Immunology and Inflammation
- Vol. 19 (2), 127-128
- https://doi.org/10.3109/09273948.2010.531175
Abstract
Purpose: To report the additional clinical value of real-time Polymerase chain reaction (PCR) in the detection of Treponema pallidum in aqueous humor. Methods: A real-time (RT) PCR assay targeting the polymerase 1 gene of Treponema pallidum was performed in aqueous humor samples collected in consecutive patients with ocular syphilis. Results: Five patients presenting with optic neuritis (1), posterior placoid chorioretinitis (1), or panuveitis with retinitis (3) were tested. The PCR results were positive in the 3 cases of panuveitis and negative in the 2 other cases. Conclusion: RT-PCR for Treponema pallidum in aqueous humor is useful to confirm syphilitic panuveitis.Keywords
This publication has 5 references indexed in Scilit:
- Clinical Value of Treponema pallidum Real-Time PCR for Diagnosis of SyphilisJournal of Clinical Microbiology, 2010
- Posterior placoid chorioretinitis: An unusual ocular manifestation of syphilisClinical Ophthalmology, 2008
- Update on ocular syphilisCurrent Opinion in Opthalmology, 2006
- Detection of Treponema pallidum in the vitreous by PCRBritish Journal of Ophthalmology, 2006
- Diagnosis of Gastric Syphilis by Direct Immunofluorescence Staining and Real-Time PCR TestingJournal of Clinical Microbiology, 2006