Expression of the chloramphenicol acetyltransferase gene inAedes albopictus(C6/36) cells using a non-infectious Sindbis virus expression vector
- 1 August 1992
- journal article
- Published by Wiley in Insect Molecular Biology
- Vol. 1 (1), 49-52
- https://doi.org/10.1111/j.1365-2583.1993.tb00077.x
Abstract
Genomic RNA was transcribed in vitro from the non-infectious Sindbis (SIN) virus expression vector (pTRCAT) and introduced into C6/36 (Aedes albopictus) cells by liposome-mediated transfections. The chloramphenicol acetyltransferase (CAT) polypeptide expressed within cells was detected by an indirect immunofluorescent assay directly into 24-well polystyrene tissue culture plates. Approximately 1 in 1000 C6/36 cells showed fluorescence when the transfection was optimized. CAT enzyme activity was also assayed; in C6/36 cells CAT expression was detected as early as 8 h after transfection, peaked at 24 h, and could still be detected at 7 days. At 24 h post-transfection each transfected C6/36 cell was calculated to express 1.3 × 107 CAT polypeptides.Keywords
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