Transducing Particles of Staphylococcus aureus Pathogenicity Island SaPI1 Are Comprised of Helper Phage-Encoded Proteins
- 15 October 2007
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 189 (20), 7520-7524
- https://doi.org/10.1128/jb.00738-07
Abstract
The relationship between the composition of SaPI1 transducing particles and those of helper phage 80α was investigated by direct comparison of virion proteins. Twelve virion proteins were identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometry; all were present in both 80α and SaPI1 virions, and all were encoded by 80α. No SaPI1-encoded proteins were detected. This confirms the prediction that SaPI1 is encapsidated in a virion assembled from helper phage-encoded proteins.Keywords
This publication has 28 references indexed in Scilit:
- Lytic Activity of Recombinant Bacteriophage φ11 and φ12 Endolysins on Whole Cells and Biofilms of Staphylococcus aureusApplied and Environmental Microbiology, 2007
- Lysis of staphylococcal mastitis pathogens by bacteriophage phi11 endolysinFEMS Microbiology Letters, 2006
- CDD: a conserved domain database for interactive domain family analysisNucleic Acids Research, 2006
- The minor capsid protein gp7 of bacteriophage SPP1 is required for efficient infection of Bacillus subtilisMolecular Microbiology, 2006
- β-Lactam Antibiotics Induce the SOS Response and Horizontal Transfer of Virulence Factors inStaphylococcus aureusJournal of Bacteriology, 2006
- Conserved Translational Frameshift in dsDNA Bacteriophage Tail Assembly GenesMolecular Cell, 2004
- Amidase domains from bacterial and phage autolysins define a family of gamma-D,L-glutamate-specific amidohydrolasesTrends in Biochemical Sciences, 2003
- The Capsid Size-determining Protein Sid Forms an External Scaffold on Phage P4 ProcapsidsJournal of Molecular Biology, 1995
- A toxic shock syndrome toxin mutant of Staphylococcus aureus isolated by allelic replacement lacks virulence in a rabbit uterine modelFEMS Microbiology Letters, 1991
- The Virion Proteins and Ultrastructure of Staphylococcus aureus BacteriophagesJournal of General Virology, 1985