DNA binding of dinuclear iron(II) metallosupramolecular cylinders. DNA unwinding and sequence preference
Open Access
- 8 May 2008
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 36 (11), 3630-3638
- https://doi.org/10.1093/nar/gkn244
Abstract
[Fe 2 L 3 ] 4+ (L = C 25 H 20 N 4 ) is a synthetic tetracationic supramolecular cylinder (with a triple helical architecture) that targets the major groove of DNA and can bind to DNA Y-shaped junctions. To explore the DNA-binding mode of [Fe 2 L 3 ] 4+ , we examine herein the interactions of pure enantiomers of this cylinder with DNA by biochemical and molecular biology methods. The results have revealed that, in addition to the previously reported bending of DNA, the enantiomers extensively unwind DNA, with the M enantiomer being the more efficient at unwinding, and exhibit preferential binding to regular alternating purine–pyrimidine sequences, with the M enantiomer showing a greater preference. Also, interestingly, the DNA binding of bulky cylinders [Fe 2 (L-CF 3 ) 3 ] 4+ and [Fe 2 (L-Ph) 3 ] 4+ results in no DNA unwinding and also no sequence preference of their DNA binding was observed. The observation of sequence-preference in the binding of these supramolecular cylinders suggests that a concept based on the use of metallosupramolecular cylinders might result in molecular designs that recognize the genetic code in a sequence-dependent manner with a potential ability to affect the processing of the genetic code.This publication has 27 references indexed in Scilit:
- Influence of surface shape on DNA binding of bimetallo helicatesJournal of Inorganic Biochemistry, 2007
- The effect of ancillary ligand chirality and phenanthroline functional group substitution on the cytotoxicity of platinum(II)-based metallointercalatorsJournal of Inorganic Biochemistry, 2007
- Recognition of DNA Three‐Way Junctions by Metallosupramolecular Cylinders: Gel Electrophoresis StudiesChemistry – A European Journal, 2007
- DNA Three-Way Junction with a Dinuclear Iron(II) Supramolecular Helicate at the Center: A NMR Structural StudyInorganic Chemistry, 2007
- Enantiomeric resolution of supramolecular helicates with different surface topographiesDalton Transactions, 2006
- Molecular Recognition of a Three‐Way DNA Junction by a Metallosupramolecular HelicateAngewandte Chemie, 2006
- Design and DNA Binding of an Extended Triple‐Stranded Metallo‐supramolecular CylinderChemistry – A European Journal, 2005
- Interaction between a DNA oligonucleotide and a dinuclear iron(II) supramolecular cylinder; an NMR and molecular dynamics studyJBIC Journal of Biological Inorganic Chemistry, 2002
- Intramolecular DNA Coiling Mediated by a Metallo-Supramolecular CylinderAngewandte Chemie, 2001
- Sequence-specific DNA binding by a two zinc-finger peptide from the Drosophila melanogaster tramtrack proteinJournal of Molecular Biology, 1992