Axonal regeneration and motor neuron survival after microsurgical nerve reconstruction
- 18 July 2012
- journal article
- research article
- Published by Wiley in Microsurgery
- Vol. 32 (7), 552-562
- https://doi.org/10.1002/micr.22036
Abstract
Rodent models are used extensively for studying nerve regeneration, but little is known about how sprouting and pruning influence peripheral nerve fiber counts and motor neuron pools. The purpose of this study was to identify fluctuations in nerve regeneration and neuronal survival over time. One hundred and forty‐four Lewis rats were randomized to end‐to‐end repair or nerve grafting (1.5 cm graft) after sciatic nerve transection. Quantitative histomorphometry and retrograde labeling of motor neurons were performed at 1, 3, 6, 9, 12, and 24 months and supplemented by electron microscopy. Fiber counts and motor neuron counts increased between 1 and 3 months, followed by plateau. End‐to‐end repair resulted in persistently higher fiber counts compared to the grafting for all time points (P < 0.05). Percent neural tissue and myelin width increased with time while fibrin debris dissipated. In conclusion, these data detail the natural history of regeneration and demonstrate that overall fiber counts may remain stable despite pruning. © 2012 Wiley Periodicals, Inc. Microsurgery, 2012.Keywords
Funding Information
- National Institutes of Health (2R01 NS33406-12, T32 DC 0022-15)
This publication has 44 references indexed in Scilit:
- Sustained Growth Factor Delivery Promotes Axonal Regeneration in Long Gap Peripheral Nerve RepairTissue Engineering, Part A, 2011
- Influence of Suture on Peripheral Nerve Regeneration and Collagen Production at the Site of Neurorrhaphy: An Experimental StudyNeurosurgery, 2011
- Costimulation blockade inhibits the indirect pathway of allorecognition in nerve allograft rejectionMuscle & Nerve, 2010
- Electrical Stimulation Accelerates Motor Functional Recovery in the Rat Model of 15-mm Sciatic Nerve Gap Bridged by Scaffolds With Longitudinally Oriented MicrochannelsNeurorehabilitation and Neural Repair, 2010
- Blockade of Nogo Receptor Ligands Promotes Functional Regeneration of Sensory Axons after Dorsal Root CrushJournal of Neuroscience, 2009
- Ephrin-B3 reverse signaling through Grb4 and cytoskeletal regulators mediates axon pruningNature Neuroscience, 2009
- Binary imaging analysis for comprehensive quantitative histomorphometry of peripheral nerveJournal of Neuroscience Methods, 2007
- Axon pruning: an essential step underlying the developmental plasticity of neuronal connectionsPhilosophical Transactions Of The Royal Society B-Biological Sciences, 2006
- Peripheral Nerve Regeneration in the Apolipoprotein-E-Deficient MouseJournal of Reconstructive Microsurgery, 2002
- Effects of sciatic nerve regeneration on axonal populations in tributary nervesBrain Research, 1984