Ameliorative Effect of Active Principle Isolated from Seeds ofEugenia jambolanaon Carbohydrate Metabolism in Experimental Diabetes
Open Access
- 1 January 2011
- journal article
- research article
- Published by Hindawi Limited in Evidence-Based Complementary and Alternative Medicine
- Vol. 2011, 1-9
- https://doi.org/10.1093/ecam/nep233
Abstract
The aim of this study was to evaluate the antidiabetic activity of LH II purified from ethanolic seed extract ofEugenia jambolanain alloxan-induced mild diabetic (MD) and severely diabetic (SD) rabbits. Ethanolic extract upon chromatographic purification yielded partially purified hypoglycemic principle (SIII) which on further purification by sephadex LH 20 yielded pharmacological active compound LH II. Homogeneity of LH II was tested by HPLC. Phytochemical investigation of LH II by various structural spectra showed the presence of saturated fatty acid, Δ5lipid and presence of sterol. LH II was administered orally at a dose of 10 mg kg−1body weight to MD and SD. LH II resulted, significant fall in FBG at 90 min (21.2% MD: 28.6% SD), 7th day (35.6% MD) and 15th day (59.6% SD). Glycosylated hemoglobin was significantly decreased (50.5%) in SD after 15 days treatment (Tt). Plasma insulin levels were significantly increased (P< .001).In vitrostudies with pancreatic islets showed 3-fold increase in insulin levels as compared to untreated animals. LH II also showed extrapancreatic effect by significantly increasing (P< .001) the activity of key enzymes of glycolysis and significantly decreasing (P< .001) the activity of key enzymes of gluconeogenesis. Liver and muscle glycogen content were increased by 36.6 and 30% for MD, and 52 and 47% for SD, respectively. Thus, the present study demonstrates that LH II possesses potent antidiabetic activity and it is effective in both MD and SD rabbits.Keywords
This publication has 41 references indexed in Scilit:
- Antidiabetic Properties ofAzardiracta indicaandBougainvillea spectabilis:In VivoStudies in Murine Diabetes ModelEvidence-Based Complementary and Alternative Medicine, 2011
- Antidiabetic Indian Plants: A Good Source of Potent Amylase InhibitorsEvidence-Based Complementary and Alternative Medicine, 2011
- Hypoglycemic and hypolipidemic effects of flavonoid rich extract from Eugenia jambolana seeds on streptozotocin induced diabetic ratsFood and Chemical Toxicology, 2008
- Red Cabbage (Brassica oleracea) Ameliorates Diabetic Nephropathy in RatsEvidence-Based Complementary and Alternative Medicine, 2008
- Antihyperglycemic effect of the fruit-pulp of Eugenia jambolana in experimental diabetes mellitusJournal of Ethnopharmacology, 2006
- Insulin-sensitizing and Anti-proliferative Effects of Argania spinosa Seed ExtractsEvidence-Based Complementary and Alternative Medicine, 2006
- Anti-Diabetic Activity ofEugenia jambolanaSeed Kernels on Streptozotocin-Induced Diabetic RatsJournal of Medicinal Food, 2004
- Insulin-like effect of vanadate on malic enzyme and glucose-6-phosphate dehydrogenase activities in streptozotocin-induced diabetic rat liverBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1991
- Induction of glucokinase by insulin under the permissive action of dexamethasone in primary rat hepatocyte culturesBiochemical and Biophysical Research Communications, 1979
- Fructose 1, 6‐Bisphosphatase: Properties of the Neutral Enzyme and its Modification by Proteolytic EnzymesPublished by Wiley ,1975