Amino acid substitutions in mitochondrial ATP synthase subunit 9 ofSaccharomyces cerevisiaeleading to venturicidin or ossamycin resistance
- 5 June 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 249 (2), 333-336
- https://doi.org/10.1016/0014-5793(89)80653-2
Abstract
A series of mitochondrially inherited mutants of yeast has been analysed, which were previously identified as showing resistance to the antibiotics venturicidin or ossamycin and whose mutations showed tight linkage to oligomycin-resistance alleles affecting subunit 9 of the mitochondrial ATP synthase. DNA sequence analysis of the oli1 gene of these mutants has been used to define the nature of amino acid substitution in the subunit 9 protein. In the case of the two venturicidin-resistant mutants, mutations affect amino acids on the N-terminal stem of the protein, namely G1y25 → Ser (venR ossS oliR) and Ala 27 → Gly (venR ossS oliS). The mutations found in the two ossamyein-resistant mutants affect amino acids on the C-terminal stem of the protein, namely Leu53 → Phe (vanS ossR oliR) and Leu57 → Phe (venS OSSR oliS). These results allow us to further develop a fine structure map of domains within the subunit 9 protein involved in antibiotic interaction.Keywords
This publication has 10 references indexed in Scilit:
- DNA sequence analysis of the Olir2-76 and Ossr1-92 alleles of the Oli-2 region of the yeast Saccharomyces cerevisiae. Analysis of related amino-acid substitutions and protein-antibiotic interactionBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1988
- Eukaryote membrane genetics: the Fo sector of mitochondrial ATP synthaseTrends in Genetics, 1988
- Amino acid substitutions in subunit 9 of the mitochondrial ATPase complex of Saccharomyces cerevisiaeEuropean Journal of Biochemistry, 1987
- Amino acid substitutions in mitochondrial ATPase subunit 6 of Saccharomyces cerevisiae leading to oligomycin resistanceFEBS Letters, 1986
- Amino acid substitutions in mitochondrial ATPase subunit 9 of saccharomyces cerevisiae leading to oligomycin or venturicidin resistanceFEBS Letters, 1986
- DNA sequence analysis of the oli1 gene reveals amino acid changes in mitochondrial ATPase subunit 9 from oligomycin‐resistant mutants of Saccharomyces cerevisiaeEuropean Journal of Biochemistry, 1985
- On the Structure and Genetics of the Proteolipid Subunit of the ATP Synthase ComplexPublished by Elsevier BV ,1981
- Identification of Amino Acid Substitutions in the Dicyclohexylcarbodiimide-Binding Subunit of the Mitochondrial ATPase Complex from Oligomycin-Resistant Mutants of Saccharomyces cerevisiaeEuropean Journal of Biochemistry, 1979
- Genetics of oxidative phosphorylation: mitochondrial loci determining ossamycin-, venturicidin- and oligomycin-resistance in yeastMolecular Genetics and Genomics, 1979
- Studies on Energy-Linked Reactions: Genetic Analysis of Venturicidin-Resistant MutantsEuropean Journal of Biochemistry, 1975