Chamber catalogues of optical and fluorescent signatures distinguish bioaerosol classes
Open Access
- 26 July 2016
- journal article
- Published by Copernicus GmbH in Atmospheric Chemistry and Physics
- Vol. 9 (7), 3283-3292
- https://doi.org/10.5194/amt-9-3283-2016
Abstract
Rapid bioaerosol characterization has immediate applications in the military, environmental and public health sectors. Recent technological advances have facilitated single-particle detection of fluorescent aerosol in near real time; this leverages controlled ultraviolet exposures with single or multiple wavelengths, followed by the characterization of associated fluorescence. This type of ultraviolet induced fluorescence has been used to detect airborne microorganisms and their fragments in laboratory studies, and it has been extended to field studies that implicate bioaerosol to compose a substantial fraction of supermicron atmospheric particles. To enhance the information yield that new-generation fluorescence instruments can provide, we report the compilation of a referential aerobiological catalogue including more than 50 pure cultures of common airborne bacteria, fungi and pollens, recovered at water activity equilibrium in a mesoscale chamber (1 m3). This catalogue juxtaposes intrinsic optical properties and select bandwidths of fluorescence emissions, which manifest to clearly distinguish between major classes of airborne microbes and pollens.This publication has 41 references indexed in Scilit:
- Evaluation of hierarchical agglomerative cluster analysis methods for discrimination of primary biological aerosolAtmospheric Chemistry and Physics, 2015
- Observations of fluorescent and biological aerosol at a high-altitude site in central FranceAtmospheric Chemistry and Physics, 2013
- A laboratory assessment of the Waveband Integrated Bioaerosol Sensor (WIBS-4) using individual samples of pollen and fungal spore materialAtmospheric Environment, 2012
- Primary biological aerosol particles in the atmosphere: a reviewTellus B: Chemical and Physical Meteorology, 2012
- Measurements and comparison of primary biological aerosol above and below a tropical forest canopy using a dual channel fluorescence spectrometerAtmospheric Chemistry and Physics, 2010
- Bacteria in the global atmosphere – Part 1: Review and synthesis of literature data for different ecosystemsAtmospheric Chemistry and Physics, 2009
- Atmospheric budget of primary biological aerosol particles from fungal sporesGeophysical Research Letters, 2009
- Thunderstorm‐asthma and pollen allergyAllergy, 2006
- Performance evaluation of the UVAPS: influence of physiological age of airborne bacteria and bacterial stressJournal of Aerosol Science, 2003
- A Combined Fluorochrome Method for Quantitation of Metabolically Active and Inactive Airborne BacteriaAerosol Science and Technology, 1999