Oscillibacter ruminantium sp. nov., isolated from the rumen of Korean native cattle
- 1 June 2013
- journal article
- Published by Microbiology Society in International Journal of Systematic and Evolutionary Microbiology
- Vol. 63 (Pt_6), 1942-1946
- https://doi.org/10.1099/ijs.0.041749-0
Abstract
A strictly anaerobic, Gram-negative, non-spore-forming bacterium, designated GH1T, was isolated from the rumen of Korean native cattle (HanWoo). Cells were straight to slightly curved rods (2.0–4.5 µm long) and were motile by peritrichous flagella. The isolate grew at 30–45 °C (optimum 40 °C), at pH 5.5–6.5 (optimum pH 6.0) and with up to 3.5 % (w/v) NaCl. Strain GH1Tproduced acid fromd-glucose,d-ribose andd-xylose, with butyric acid being the major end product. The genomic DNA G+C content was 54.6 mol%. Based on comparative 16S rRNA gene sequence analysis, strain GH1Twas most closely related to Oscillibacter valericigenes Sjm18-20T(97.3 % 16S rRNA gene sequence similarity). DNA–DNA hybridization between strain GH1Tand O. valericigenes DSM 18026Tshowed 24 % reassociation. The major fatty acids were iso-C13 : 0(13.0 %), iso-C15 : 0(17.6 %), anteiso-C15 : 0(8.4 %) and C14 : 0(4.1 %), and the cellular fatty acid methyl esters as dimethylacetals (DMAs) were C16 : 0DMA (17.8 %), iso-C15 : 0DMA (15.2 %) and C14 : 0DMA (4.52 %). The cell-wall peptidoglycan of strain GH1Tcontainedmeso-diaminopimelic acid and the major cell-wall sugar was galactose. Based on 16S rRNA gene sequence similarity, phylogenetic analysis, DNA G+C content, DNA–DNA relatedness and distinct phenotypic characteristics, strain GH1Tis classified in the genus Oscillibacter as a member of a novel species, for which the name Oscillibacter ruminantiumsp. nov. is proposed. The type strain is GH1T( = KCTC 15176T = NBRC 108824T = JCM 18333T).Keywords
Funding Information
- KRIBB Research Initiative Program
- Korea government (MEST (NMM0101232)
- National Research Foundation of Korea (OGM0211212)
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