Impact of Lifelong Sedentary Behavior on Mitochondrial Function of Mice Skeletal Muscle
Open Access
- 22 May 2009
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journals of Gerontology: Series A
- Vol. 64A (9), 927-939
- https://doi.org/10.1093/gerona/glp066
Abstract
This study investigated the impact of lifelong sedentariness on skeletal muscle mass and mitochondrial function. Thirty C57BL/6 strain mice (2 months) were randomly divided into three groups (young-Y; old sedentary-OS; old active-OA). Young animals were sacrificed after 1 week of quarantine, and OS and OA groups were individually placed into standard cages and in cages with running wheels, respectively, until sacrifice (25 months). Body weights and hind-limb skeletal muscle wet weights were obtained from all groups. Mitochondrial respiratory functional measures (i.e., state 3 and 4 respiration, respiratory control ratio, and ratio of nanomoles of ADP phosphorylated by nanomoles of O2 consumed [ADP/O]) and biochemical markers of oxidative damage (aconitase activity, protein carbonyl derivatives, sulfhydryl groups) were measured in isolated mitochondrial suspensions. Our results reveal that lifelong sedentary behavior has a negative impact on the age-related loss of skeletal muscle mass and on the isolated mitochondrial function of mixed skeletal muscle of mice, which is associated with an increased oxidative damage to mitochondrial biomolecules.Keywords
This publication has 69 references indexed in Scilit:
- Aging Impairs Skeletal Muscle Mitochondrial Bioenergetic FunctionThe Journals of Gerontology: Series A, 2009
- Lifelong exercise and mild (8%) caloric restriction attenuate age-induced alterations in plantaris muscle morphology, oxidative stress and IGF-1 in the Fischer-344 ratExperimental Gerontology, 2008
- The role of mitochondria in aging of skeletal muscleBiogerontology, 2008
- Exercise by lifelong voluntary wheel running reduces subsarcolemmal and interfibrillar mitochondrial hydrogen peroxide production in the heartAmerican Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 2005
- Acute and severe hypobaric hypoxia increases oxidative stress and impairs mitochondrial function in mouse skeletal muscleJournal of Applied Physiology, 2005
- The effect of ageing and immobilization on structure and function of human skeletal muscle fibresThe Journal of Physiology, 2003
- Mitochondrial function and antioxidative defence in human muscle: effects of endurance training and oxidative stressThe Journal of Physiology, 2000
- Effect of Training on H2O2 Release by Mitochondria from Rat Skeletal MuscleArchives of Biochemistry and Biophysics, 1999
- Effects of endurance training on oxidative capacity and structural composition of human arm and leg musclesActa Physiologica Scandinavica, 1997
- Effect of exercise training on insulin binding and glucose metabolism in mouse soleus muscleCanadian Journal of Physiology and Pharmacology, 1987