Induction of formation of presynaptic terminals in neuroblastoma cells by synapsin IIb
- 1 February 1991
- journal article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 349 (6311), 697-700
- https://doi.org/10.1038/349697a0
Abstract
The synapsins are a family of closely related phosphoproteins (termed synapsins Ia, Ib, IIa and IIb) associated with synaptic vesicles and implicated in the short-term regulation of neurotransmitter release from nerve endings. During development, expression of the synapsins correlates temporally with synapse formation, but there has been no direct evidence that they are involved in synaptogenesis. Here we report that overexpression of synapsin IIb in the neuroblastoma x glioma hybrid clonal cell line NG108-15 leads, during cell differentiation, to marked increases in the number of neuritic varicosities and in the numbers of small clear vesicles and large dense core vesicles per varicosity, as well as to the appearance of synapse-like cell-cell contacts. Thus, synapsin IIb may be involved in the regulation of synapse formation and, as a result, in long-term neuronal signalling.Keywords
This publication has 16 references indexed in Scilit:
- The synapsins and the regulation of synaptic functionBioEssays, 1990
- Synapsins: Mosaics of Shared and Individual Domains in a Family of Synaptic Vesicle PhosphoproteinsScience, 1989
- The cell biology of the nerve terminalNeuron, 1988
- Protein p38: an integral membrane protein specific for small vesicles of neurons and neuroendocrine cells.The Journal of cell biology, 1986
- Nerve growth factor does not activate Na+/H+ exchange in PC12 pheochromocytoma cellsJournal of Cellular Physiology, 1985
- Modulation of Synapse Formation by Cyclic Adenosine MonophosphateScience, 1983
- Ultrastructural changes in differentiating neuroblastoma × glioma hybrid cellsTissue and Cell, 1983
- A Diurnal Rhythm in Pineal Protein 1 Content Mediated by β-Adrenergic NeurotransmissionScience, 1982
- Corticosterone Increases the Amount of Protein 1, A Neuron-Specific Phosphoprotein, in Rat HippocampusScience, 1981
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970