Secreted antigens of filarial nematodes: a survey and characterization of in vitro excreted/secreted products of adult Brugia malayi

Abstract

Summary We report here a broad analysis of the excretory/secretory (E/S) products of adult Brugia malayi, collected by in–vitro cultivation of the parasite. Culture media and conditions were optimized, and non–essential amino acids were found to be crucial for efficient protein synthesis under cell– and serum–free culture conditions. A close correlation was found between total protein secretion, phosphorylcholine–bearing antigen release and lactate production on each day of culture, indicating that E/S molecules are actively secreted. Parasites cultured in vitro take 2–3 days to adjust to the new environment, and show peak levels of secretion at days 3 and 4. The active secretion of phosphorylcholine by the parasite therefore justifies the measurement of this molecule as an indication of active infection, possibly reflecting total worm burdens. By comparing metaboli–cally labelled E/S from male and female worms, several molecules of low mol. wt, namely 10000, 13000, 14 000 and 22 000, together with high mol. wt components of above 12000 were found to be female specific. Tracing the origin of the E/S products, several molecules were also found to be associated with the surface. Among these, there are at least two glycoproteins, 29 000 and 51000 of which the 29000 molecule is a major surface protein. The immunogenicity of the E/S was examined and antigenic cross–reactivity was found with sera from most filarial infections but not with non–filarial nematodiases such as hookworm or Trichi–. nella. However, two molecules of low mol. wt, 15 000 and 19000, were not recognized by anti–Onchocerca sera and appeared to be potential Brugia–specific diagnostic molecules. Possible functional roles of the adult E/S products were examined but we could find no evidence of protease activity in the E/S or glutathione S–transferase activity in either the E/S or in whole somatic extract.