Floral organ abscission peptide IDA and its HAE/HSL2 receptors control cell separation during lateral root emergence
Open Access
- 11 March 2013
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 110 (13), 5235-5240
- https://doi.org/10.1073/pnas.1210835110
Abstract
Throughout their life cycle, plants produce new organs, such as leaves, flowers, and lateral roots. Organs that have served their purpose may be shed after breakdown of primary cell walls between adjacent cell files at the site of detachment. In Arabidopsis, floral organs abscise after pollination, and this cell separation event is controlled by the peptide INFLORESCENCE DEFICIENT IN ABSCISSION (IDA), which signals through the leucine-rich repeat receptor-like kinases HAESA (HAE) and HAESA-LIKE2 (HSL2). Emergence of new lateral root primordia, initiated deep inside the root under the influence of auxin, is similarly dependent on cell wall dissolution between cells in the overlaying endodermal, cortical, and epidermal tissues. Here we show that this process requires IDA, HAE, and HSL2. Mutation in these genes constrains the passage of the growing lateral root primordia through the overlaying layers, resulting in altered shapes of the lateral root primordia and of the overlaying cells. The HAE and HSL2 receptors are redundant in function during floral organ abscission, but during lateral root emergence they are differentially involved in regulating cell wall remodeling genes. In the root, IDA is strongly auxin-inducible and dependent on key regulators of lateral root emergence—the auxin influx carrier LIKE AUX1-3 and AUXIN RESPONSE FACTOR7. The expression levels of the receptor genes are only transiently induced by auxin, suggesting they are limiting factors for cell separation. We conclude that elements of the same cell separation signaling module have been adapted to function in different developmental programs.Keywords
This publication has 35 references indexed in Scilit:
- A Novel Approach to Dissect the Abscission Process in ArabidopsisPlant Physiology, 2012
- Casparian strip diffusion barrier in Arabidopsis is made of a lignin polymer without suberinProceedings of the National Academy of Sciences of the United States of America, 2012
- Propidium Iodide Competes with Ca2+ to Label Pectin in Pollen Tubes and Arabidopsis Root HairsPlant Physiology, 2011
- ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE1 (ADPG1), ADPG2, and QUARTET2 Are Polygalacturonases Required for Cell Separation during Reproductive Development inArabidopsisPlant Cell, 2009
- Regulation of floral organ abscission in Arabidopsis thalianaProceedings of the National Academy of Sciences of the United States of America, 2008
- The EPIP Peptide of INFLORESCENCE DEFICIENT IN ABSCISSION Is Sufficient to Induce Abscission inArabidopsisthrough the Receptor-Like Kinases HAESA and HAESA-LIKE2Plant Cell, 2008
- Stamen Abscission Zone Transcriptome Profiling Reveals New Candidates for Abscission Control: Enhanced Retention of Floral Organs in Transgenic Plants Overexpressing ArabidopsisZINC FINGER PROTEIN2Plant Physiology, 2008
- The Arabidopsis Unannotated Secreted Peptide Database, a Resource for Plant PeptidomicsPlant Physiology, 2006
- Receptor-like kinases from Arabidopsis form a monophyletic gene family related to animal receptor kinasesProceedings of the National Academy of Sciences of the United States of America, 2001
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962