The effects of zero (ZT), minimum (MT) and conventional tillage (CT) systems and three 4-yr crop rotations on soil total C and N, mineralizable N, NO3-N, P, K and SO4-S accumulation and distribution in the soil profile were determined after one 4-yr cycle of crop production on Black Chernozemic Indian Head heavy clay soil. The distributions of available P and K in the profile were not affected by tillage or rotation. Total C and N in the 0–5 cm depth was higher in the reduced tillage systems than in CT, but differences in the 5–10 cm and 10–15 cm depths were not significant. Nitrate concentration was higher in the 15–30 cm and 30–60 cm depths under CT than under reduced tillage. Inclusion of fallow increased accumulation of NO3-N in the deeper soil depths, while inclusion of winter wheat in the rotation reduced deep movement of NO3-N. Nitrate-nitrogen accumulation after field pea production was comparable to that after winter wheat, but a greater proportion of the NO3-N was present near the soil surface after winter wheat, reducing the potential for leaching below the rooting zone. Potential for movement of NO3-N below the rooting zone may be increased by fallow and decreased by production of winter wheat and by reduced tillage systems. Key words: Tillage, rotations, nitrate, sulphate