Strigolactone regulation of shoot branching in chrysanthemum (Dendranthema grandiflorum)
Open Access
- 17 May 2010
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 61 (11), 3069-3078
- https://doi.org/10.1093/jxb/erq133
Abstract
Previous studies of highly branched mutants in pea (rms1–rms5), Arabidopsis thaliana (max1–max4), petunia (dad1–dad3), and rice (d3, d10, htd1/d17, d14, d27) identified strigolactones or their derivates (SLs), as shoot branching inhibitors. This recent discovery offers the possibility of using SLs to regulate branching commercially, for example, in chrysanthemum, an important cut flower crop. To investigate this option, SL physiology and molecular biology were studied in chrysanthemum (Dendranthema grandiflorum), focusing on the CCD8/MAX4/DAD1/RMS1/D10 gene. Our results suggest that, as has been proposed for Arabidopsis, the ability of SLs to inhibit bud activity depends on the presence of a competing auxin source. The chrysanthemum SL biosynthesis gene, CCD8 was cloned, and found to be regulated in a similar, but not identical way to known CCD8s. Expression analyses revealed that DgCCD8 is predominantly expressed in roots and stems, and is up-regulated by exogenous auxin. Exogenous SL can down-regulate DgCCD8 expression, but this effect can be overridden by apical auxin application. This study provides evidence that SLs are promising candidates to alter the shoot branching habit of chrysanthemum.Keywords
This publication has 57 references indexed in Scilit:
- Control of bud activation by an auxin transport switchProceedings of the National Academy of Sciences of the United States of America, 2009
- Strigolactone Acts Downstream of Auxin to Regulate Bud Outgrowth in Pea and ArabidopsisPlant Physiology, 2009
- The Sorghum bicolor genome and the diversification of grassesNature, 2009
- A Rapid Bootstrap Algorithm for the RAxML Web ServersSystematic Biology, 2008
- Genome Structure of the Legume, Lotus japonicusDNA Research, 2008
- The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phylaNature, 2007
- The TIGR Rice Genome Annotation Resource: improvements and new featuresNucleic Acids Research, 2006
- RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed modelsBioinformatics, 2006
- MUSCLE: multiple sequence alignment with high accuracy and high throughputNucleic Acids Research, 2004
- The Arabidopsis Information Resource (TAIR): a model organism database providing a centralized, curated gateway to Arabidopsis biology, research materials and communityNucleic Acids Research, 2003